DESIGN OF COLORIMETRIC AND CHIROPTICAL SENSORS FOR ABUSED NARCOTICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在首席研究员、化学助理教授安德里亚·霍姆斯的指导下，多恩大学的本科生 学院将参与一个正在进行的研究项目，以开发新的高选择性和高灵敏度的分子传感器，在非法毒品存在的情况下改变颜色。将使用组合方法来寻找这些传感器，通常 滥用药物，如可卡因，约会强奸药物，如伦硝西潘和GHB，以及甲基苯丙胺。该项目涉及使用现代分析技术，例如通过UV/Vis和荧光光谱测定结合常数， 使用基于寡核苷酸的结合体(适配子)和组合96孔板方法，从而使本科生化学研究与当前使用的主流技术相结合。在五年的研究和教育期间，预期的里程碑是确定与SELEX使用可卡因已获得的适配子形成强烈络合物的更好的染料，通过使用固定化氟硝西潘和SELEX找到更具体的适配子并测试这些染料，将方法学扩展到其他滥用麻醉品，如GHB和甲基苯丙胺，并将传感器添加到现场的固体支持 由执法部门和卫生专业人员进行检测。所有的工作都将完全可见，并与教育 多恩化学项目的目标。 智力优势：该项目依赖于物理、有机、分析和 法医化学。教师/本科生研究团队将利用创造性和原创性的组合技术， 使用适配子和固相技术实现简单的“混合测量”传感分析，有可能成为 扩大到其他被滥用的麻醉药品，因此与人类健康高度相关。丰富的学习环境 研究，更好的教学材料，协作学习，跨学科研究，并融入现代 工具被认为是赋予学生权力和有效学习科学的一些最重要的关键 教育。 更广泛的影响：如果成功，这项研究将导致开发新的、强大的、敏感的和选择性的分子 用于手持式比色现场测试的传感器，可供执法、卫生专业人员、分析人员使用 实验室、法医科学家和药物攻击或过量服药的潜在受害者。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Under direction of principal investigator Andrea Holmes, assistant professor of chemistry, undergraduate students at Doane College will participate in an ongoing research project to develop new highly selective and sensitive molecular sensors that change color in the presence of illegal narcotics. A combinatorial approach will be used to find these sensors for commonly abused drugs such as cocaine, date-rape drugs like lunitrazepam and GHB, and methamphetamine. The project involves the use of modern analytical techniques, such as the determination of binding constants by UV/Vis and fluorescence spectroscopy, work with oligonucleotide-based binders (aptamers), and combinatorial 96-well plate methods, thus allowing the integration of undergraduate chemistry research with currently used mainstream technology. Anticipated milestones for the five-year period of research and education are to determine better dyes that form strong complexes with aptamers already obtained by SELEX using cocaine, find more specific aptamers by using immobilized flunitrazepam and SELEX and test the dyes, expand the methodology to other abused narcotics such as GHB and methamphetamine, and add the sensor to solid support for field testing by law enforcement and health professionals. All the work will be fully visible and integrated with the educational goals of the Doane chemistry program. Intellectual Merit: The project relies on the synergism of the interdisciplinary fields of physical, organic, analytical, and forensic chemistry. The faculty/undergraduate research team will utilize creative and original combinatorial techniques that lead to simple "mix and measure" sensing assays using aptamer and solid-phase technology that have the potential to be expanded to other abused narcotics and is therefore highly relevant to human health. Learning environments that are rich in research, better instructional materials, collaborative learning, interdisciplinary research, and incorporation of modern instruments are recognized as some of the most important keys to student empowerment and effective learning in science education. Broader Impact: If successful, the research will lead to the development of novel, robust, sensitive, and selective molecular sensors for use as hand-held colorimetric field-tests that could be used by law enforcement, health professionals, analytical laboratories, forensic scientists, and potential victims of drug assault or overdosing.