DESIGN OF COLORIMETRIC AND CHIROPTICAL SENSORS FOR ABUSED NARCOTICS

用于滥用麻醉品的比色和手光传感器的设计

• 批准号: 7960283
• 负责人: ANDREA E HOLMES
• 金额: $ 2.1万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960283
• 关键词: Binding; Biological Assay; Chemistry; Cocaine; Color; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Dyes; Education; Environment; Faculty; Flunitrazepam; Fluorescence Spectroscopy; Forcible intercourse; Forensic Medicine; Funding; Goals; Grant; Hand; Health; Health Professional; Human; Institution; Interdisciplinary Study; Laboratories; Law Enforcement; Lead; Learning; Mainstreaming; Measures; Methamphetamine; Methodology; Methods; Molecular; Narcotic Abuses; Narcotics; Nebraska; Oligonucleotides; Overdose; Pharmaceutical Preparations; Phase; Principal Investigator; Research; Research Personnel; Research Project Grants; Resources; Scientist; Solid; Source; Students; Techniques; Technology; Testing; United States National Institutes of Health; Work; aptamer; assault; base; cocaine use; college; combinatorial; design; drug of abuse; empowerment; functional genomics; instrument; novel; professor; programs; science education; sensor; synergism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Under direction of principal investigator Andrea Holmes, assistant professor of chemistry, undergraduate students at Doane College will participate in an ongoing research project to develop new highly selective and sensitive molecular sensors that change color in the presence of illegal narcotics. A combinatorial approach will be used to find these sensors for commonly abused drugs such as cocaine, date-rape drugs like lunitrazepam and GHB, and methamphetamine. The project involves the use of modern analytical techniques, such as the determination of binding constants by UV/Vis and fluorescence spectroscopy, work with oligonucleotide-based binders (aptamers), and combinatorial 96-well plate methods, thus allowing the integration of undergraduate chemistry research with currently used mainstream technology. Anticipated milestones for the five-year period of research and education are to determine better dyes that form strong complexes with aptamers already obtained by SELEX using cocaine, find more specific aptamers by using immobilized flunitrazepam and SELEX and test the dyes, expand the methodology to other abused narcotics such as GHB and methamphetamine, and add the sensor to solid support for field testing by law enforcement and health professionals. All the work will be fully visible and integrated with the educational goals of the Doane chemistry program. Intellectual Merit: The project relies on the synergism of the interdisciplinary fields of physical, organic, analytical, and forensic chemistry. The faculty/undergraduate research team will utilize creative and original combinatorial techniques that lead to simple "mix and measure" sensing assays using aptamer and solid-phase technology that have the potential to be expanded to other abused narcotics and is therefore highly relevant to human health. Learning environments that are rich in research, better instructional materials, collaborative learning, interdisciplinary research, and incorporation of modern instruments are recognized as some of the most important keys to student empowerment and effective learning in science education. Broader Impact: If successful, the research will lead to the development of novel, robust, sensitive, and selective molecular sensors for use as hand-held colorimetric field-tests that could be used by law enforcement, health professionals, analytical laboratories, forensic scientists, and potential victims of drug assault or overdosing.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在首席研究员Andrea Holmes的指导下，Doane的本科生化学助理教授 大学将参加一个正在进行的研究项目，以开发新的高度选择性和敏感的分子传感器，这些传感器在存在非法麻醉品的情况下会改变颜色。组合方法将用于查找这些传感器通常 滥用的药物，例如可卡因，lunitrazepam和GHB等枣强奸药物，以及甲基苯丙胺。该项目涉及现代分析技术的使用，例如通过紫外线/VIS和荧光光谱确定结合常数， 与基于寡核苷酸的粘合剂（APTAMERS）和组合96孔板方法一起工作，从而可以将本科化学研究与当前使用的主流技术整合在一起。在五年的研究和教育期间，预期的里程碑是为了确定更好的染料，该染料与已经使用可卡因获得的SELEX已经获得的适体形成了强大的染料，通过使用固定化的氟尼替氏酶和SELEX来查找更具体的适体，并测试染料，并测试染料，将方法扩展到其他滥用的毒品，例如GHB和GHB和甲基甲基和甲基甲胺和甲基甲胺和甲基甲胺和甲基甲烷和甲基甲烷和甲基苯丙胺，并将其扩展。 执法和卫生专业人员的测试。所有工作将完全可见，并与教育 Doane化学计划的目标。 智力优点：该项目依赖于物理，有机，分析和分析的跨学科领域的协同作用 法医化学。教师/本科研究团队将利用创意和原始组合技术 使用适体和固相技术导致简单的“混合和测量”传感测定法，这些技术有潜力 扩展到其他受虐待的麻醉品，因此与人类健康高度相关。丰富的学习环境 研究，更好的教学材料，协作学习，跨学科研究以及现代的融合 仪器被认为是赋予学生能力和有效学习的一些最重要的关键 教育。 更广泛的影响：如果成功，研究将导致新颖，鲁棒，敏感和选择性分子的发展 用作手持比色测试的传感器，执法，卫生专业人员，分析 实验室，法医科学家以及毒品攻击或过量药物的潜在受害者。