UKY DENTAL COBRE: ORAL INFECTIONS: IMPACT ON GESTATIONAL DIABETES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们的一般假设是，GDM女性比非GDM女性发生更严重牙周病的风险更高，GDM和牙周病的组合将与对孕产妇和胎儿健康的负面影响增加有关。 这些前瞻性临床数据将与基础科学实验室对微生物菌群和宿主反应的评价相关联，以深入了解导致与炎症性牙周病和GDM相关的不良母儿结局的致病机制。 该项目包括三个具体目标，旨在研究妊娠糖尿病（GDM）对牙周病的临床，微生物学和免疫学特征的影响以及这些潜在危险因素与母亲和胎儿健康之间的关系。 在入组时，研究者进行临床牙周检查，以记录牙周炎的存在和严重程度（具体目标1）。 在妊娠早期（31周）入组的患者可能仅接受一次访视。 对没有GDM的对照进行可比检查，所述对照在年龄、种族/民族和孕周方面与测试受试者匹配。 受试者根据糖尿病状态和有无牙周炎分为四组  GDM + PD组（GDM + PD组）：GDM + PD组（GDM + PD组  PD组：正常对照组（NC + PD）; 4组：正常对照组（NC  PD）。 除了进行临床检查外，还收集牙菌斑和血清样品作为基线和34周检查的一部分，使我们能够表征龈下生物膜微生物群（特定目标2）并量化每组和每个时间点的全身炎症生物标志物（特定目标3）的水平。研究人群包括一组年龄在16-45岁之间的非卧床妇女，她们的妊娠由肯塔基州大学诊所、Gordon and Salter，Inc.在里士满，肯塔基州和乔治敦妇产科实践乔治敦，肯塔基州。 我们目前招募了271例受试者，分为以下类别：GDM + PD 56; GDM + PD 56; GDM + PD 56; GDM + PD 56; GDM + PD 56。  PD 83; NC + PD 42; NC  PD 90。 族裔/种族概况为100名白色人、120名西班牙裔/拉丁美洲人、29名黑人/非裔美国人和22名亚洲人。 所有的数据，包括母亲和胎儿的结果，已经收集了230这些受试者。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our GENERAL HYPOTHESIS is that women with GDM are at higher risk for developing more severe periodontal disease than women without GDM and that the combination of GDM and periodontal disease will be associated with an increased negative impact on maternal and fetal health. These prospective clinical data will be correlated with basic science laboratory evaluations of the microflora and host response to provide insight into the pathogenic mechanisms contributing to negative maternal and fetal outcomes associated with inflammatory periodontal disease and GDM. This project consists of three specific aims that are designed to study the effects of Gestational Diabetes Mellitus (GDM) on the clinical, microbiologic and immunologic characteristics of periodontal disease and the associations between these potential risk factors and maternal and fetal health. At enrollment, the investigator performs a clinical periodontal examination to document the presence and severity of periodontitis (Specific Aim 1). Patients who are enrolled early in their pregnancy (31 weeks), may only be seen for one visit. Comparable examinations are performed on the controls without GDM, who have been matched with the test subjects for age, race/ethnicity and gestational week. The subjects are stratified into four groups based on diabetic status and the presence or absence of periodontitis  Group 1: GDM with periodontitis (GDM + PD); Group 2: GDM without periodontitis (GDM  PD); Group 3: normal controls with periodontitis (NC + PD); and Group 4: normal controls without periodontitis (NC  PD). In addition to performing the clinical examinations, dental plaque and serum samples are collected as part of the baseline and 34 week examinations, allowing us to characterize the subgingival biofilm microbiota (Specific Aim 2) and quantify levels of systemic inflammatory biomarkers (Specific Aim 3) in each group and at each time point. The study population consists of a group of ambulatory women, aged 16-45, whose pregnancies are being managed through the University of Kentucky Clinics, the Gordon and Salter, Inc. practice in Richmond, KY and the Georgetown Obstetrics and Gynecology practice in Georgetown, KY. We have currently enrolled 271 subjects into the following categories: GDM + PD 56; GDM  PD 83; NC + PD 42; NC  PD 90. The ethnic/racial profiles are 100 White, 120 Hispanic/Latino, 29 Black/African American and 22 Asian. All of the data, including maternal and fetal outcomes, have been collected on 230 of these subjects.