COBRE: U OF KANSAS MEDICAL CTR: CORE D: HISTOLOGY/PHENOTYPING CORE

COBRE：堪萨斯大学医学 CTR：核心 D：组织学/表型核心

• 批准号: 7720182
• 负责人: Bryan L Copple
• 金额: $ 3.31万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720182
• 关键词: Centers of Research Excellence; Characteristics; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Condition; Diet; Fatty Liver; Funding; Gene Expression; Grant; Histology; In Situ Hybridization; Institution; Kansas; Knockout Mice; Liver; Localized; Measures; Medical; Mus; Pharmaceutical Preparations; Phenotype; Proteins; Research; Research Personnel; Resources; Serum; Source; Techniques; Tissues; Transgenic Organisms; United States National Institutes of Health; fibrogenesis; immunocytochemistry; injury and repair

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Phenotyping Core will support the COBRE projects by facilitating the rapid phenotypic analysis of transgenic or null mice. This Core will do histopathological and immunocytochemistry analysis of livers and serum chemistry of null mice, compared to control mice, as well as under different conditions (diet, drugs, etc). The functions of the Core will include determining gross histological characteristics of tissue, measuring and localizing protein and gene expression in liver or other tissues by immunocytochemistry or in situ hybridization techniques, and performing serum chemistry analysis to follow liver injury and repair during studies of fibrogenesis or fatty liver.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 表型核心将通过促进转基因或空白小鼠的快速表型分析来支持Cobre项目。该中心将对空白小鼠的肝脏和血清化学进行组织病理学和免疫细胞化学分析，与对照小鼠相比，以及在不同条件下(饮食、药物等)。 核心的职能将包括确定组织的大体组织学特征，通过免疫细胞化学或原位杂交技术测量和定位肝脏或其他组织中的蛋白质和基因表达，以及在纤维化或脂肪肝研究期间进行血清化学分析，以跟踪肝脏损伤和修复。