LIGAND-RECEPTOR SYSTEMS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们已经开始为流式细胞术检查珠子上显示的受体和信号分子(Nolan和Sklar，1998；Sklar等人)。2000)。我们已经展示了His标记的受体可以与Ni2+珠子结合并结合配体的条件。我们还表明，溶解的受体可以与异三聚体G蛋白(NeuBig的亚基)和阻滞素(Prossnitz)重组以用于荧光，并有可能用于流式细胞仪分析。我们已经尝试使用arrestin、受体以及生物素化受体尾肽作为珠子上的诱饵。我们正在检测生物素化的抗His抗体，而不是螯合的Ni2+珠子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have begun to examine both receptors and signaling molecules displayed on beads for flow cytometry (Nolan and Sklar, 1998; Sklar et al. 2000). We have shown conditions under which his-tagged receptors may be bound to Ni2+ beads and bind ligand. We have also shown that solublized receptors may be reconstituted with heterotrimeric G proteins (subunits from Neubig) and arrestins (Prossnitz) for fluorescence, and potentially for flow cytometric analysis. We have attempted to use arrestin, receptors, as well as biotinylated receptor tail peptides as bait on beads. We are examining biotinylated anti-his antibodies instead of chelated Ni2+ beads.