HIGH THROUGHPUT, HIGH CONTENT MOLECULAR LIBRARIES SCREENING

高通量、高内涵分子库筛选

• 批准号: 8361764
• 负责人: LARRY A. SKLAR
• 金额: $ 2.24万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361764
• 关键词: Acoustics; Address; Binding; Biological Assay; Cells; Collaborations; Computer software; Cytometry; Flow Cytometry; Fluorescence; Funding; Goals; Grant; Libraries; Luminescent Measurements; Measures; Molecular; Molecular Bank; National Center for Research Resources; New Mexico; Principal Investigator; Recording of previous events; Research; Research Infrastructure; Resources; Screening procedure; Sorting - Cell Movement; Source; System; United States National Institutes of Health; Universities; abstracting; base; combinatorial; cost; data acquisition; digital; improved; instrument; particle; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Abstract The application of flow cytometry as a high-throughput, high information content screening platform is just beginning and has not reached its full potential. The NFCR and the University of New Mexico cytometry group will extend their long and productive history of collaboration into the technical advancement and improved application of flow-based molecular screening assays. This collaboration will extend across each of the three R&D Projects, and will evolve with the instrumental advances developed as these projects progress. In Project 1, we will use the new technical capabilities of the acoustic-focusing instrument to extend the range of flow-based screening assays into the realm of luminescence measurements. In Project 2, we will use the large-particle sorter to develop improved combinatorial library screening. In Project 3, we will develop improved multiplexed and molecular interaction screening assays based on the ability to simultaneously measure complete emission spectra and fluorescence lifetime. We will also continue our ongoing collaboration to apply the advantages of the NFCR-developed Open Reconfigurable Cytometry Acquisition System (ORCAS) digital data acquisition hardware/software to flow screening instruments developed by the UNM group. This collaboration has the potential to considerably extend the throughput, range and information content of a wide variety of cell-based and particle-based flow screening assays, directly addressing major goals of the NIH Roadmap Initiative.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和子项目主要研究者的主要支持可能由其他来源提供，包括其他NIH来源。 为子项目列出的总成本可能代表子项目使用的中心基础设施的估计数量，而不是NCRR赠款向子项目或子项目工作人员提供的直接资金。 摘要 流式细胞术作为高通量、高信息量筛选平台的应用才刚刚开始，尚未充分发挥其潜力。 NFCR和新墨西哥州大学细胞计数组将把他们长期富有成效的合作历史扩展到技术进步和基于流动的分子筛选测定的改进应用中。 这种合作将扩展到三个研发项目中的每一个，并将随着这些项目的进展而发展。 在项目1中，我们将使用声聚焦仪器的新技术能力，将基于流动的筛选测定的范围扩展到发光测量领域。 在项目2中，我们将使用大颗粒分选仪来开发改进的组合文库筛选。 在项目3中，我们将开发基于同时测量完整发射光谱和荧光寿命的能力的改进的多重和分子相互作用筛选测定。 我们还将继续我们正在进行的合作，将NFCR开发的开放式可重构细胞计数采集系统（ORCAS）数字数据采集硬件/软件的优势应用于UNM集团开发的流动筛选仪器。 这种合作有可能大大扩展各种基于细胞和基于颗粒的流动筛选测定的通量、范围和信息内容，直接解决NIH路线图倡议的主要目标。