Autophagy Scientific Core

自噬科学核心

• 批准号: 10249118
• 负责人: LARRY A. SKLAR
• 金额: $ 23.99万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10249118
• 关键词: Area; Autophagocytosis; Award; Biological Assay; Biological Models; Centers of Research Excellence; Consultations; Continuing Education; Core Facility; Data; Data Analyses; Development; Disease; Education; Educational workshop; Ensure; Equipment; Etiology; Event; Experimental Designs; Face; Fostering; Funding; Future; Genotype; Goals; Health; Health Sciences; Homeostasis; Human Resources; Image; Inflammation; Inflammatory; Infrastructure; Interdisciplinary Study; Intervention; Investigation; Knowledge; Lead; Leadership; Learning; Link; Measures; Mentors; Metabolic; Metabolic Diseases; Metabolic dysfunction; Metabolism; Mission; Molecular; New Mexico; Pathogenicity; Phase; Phenotype; Principal Investigator; Process; Publications; Quality Control; Regulation; Research; Research Design; Research Personnel; Research Support; Resources; Role; Science; Services; Side; System; Techniques; Training; Training Support; Transgenic Animals; Transgenic Model; United States National Institutes of Health; Universities; animal colony; cohort; falls; in vivo; innovation; insight; instrumentation; interest; multidisciplinary; outreach; programs

SUMMARY In support of the Autophagy, Inflammation and Metabolism (AIM) Center of Biomedical Research Excellence (CoBRE), the Autophagy Science Core (ASC) will centralize expertise, training, and instrumentation for high quality autophagy assays. Although the University of New Mexico Health Science Center (UNM HSC) enjoys a breadth of investigator expertise in various disease states, a centralized facility is needed to support autophagy investigations. The ASC facility will enable AIM mentored PIs (mPIs) and a growing cadre of investigators at UNM HSC, statewide, and regionally to implement autophagy assays into their research. In support of the AIM CoBRE mission, these centralized ASC resources will accelerate the mPI trajectory toward independent NIH- R01 level funding, thus, side-stepping the steep learning curve mPIs typically face. ASC will share a newly remodeled space—the scientific cores lab (SCICORE lab)—and will interface with the AIM Inflammation and Metabolism Core. For different applications the two scientific cores will share an AMNIS ImageStream X Mark II flow cytometer and will jointly participate in its upgrades, applications, development and oversight through the expertise of a single technical support staff. Also ASC will maintain an autophagy transgenic animal colony with expert genotyping and phenotype quality control. ASC will not duplicate the existing shared equipment and will fully interface with existing facilities at UNM HSC and with existing cores at other Institutional Development Award (IDeA) centers in New Mexico. Furthermore, ASC will foster multidisciplinary interactions between established and junior investigators at UNM HSC and regionally by supporting research in a wide range of autophagy-implicated fundamental processes and disease states. The following specific aims will be accomplished: Specific Aim 1. Create a unique single resource to provide both expertise and experimental capabilities in autophagy techniques and research Specific Aim 2. Enable and enhance the autophagy research of mentored PIs and develop the new cohorts of CoBRE investigators to study autophagy in the contexts of inflammation and metabolism. Specific Aim 3. Lead, coordinate, and develop autophagy research across UNM HSC and the state. Achieving these aims will facilitate R01 attainment of the current roster of mPIs; develop a new cadre of investigators using autophagy measures; establish a long-term sustainable core to enable AIM research; and support research at UNM HSC, statewide, and in regional IDeA states. ASC, and by extension AIM, will become a national resource through technical workshops and other modes of dissemination.

总结 自噬，炎症和代谢（AIM）卓越生物医学研究中心 自噬科学核心（ASC）将集中专业知识，培训和仪器， 高质量的自噬测定。虽然新墨西哥州大学健康科学中心（UNM HSC）享有 由于研究者在各种疾病状态下拥有广泛的专业知识，因此需要一个集中的设施来支持自噬 调查事务所ASC设施将使AIM指导的PI（mPI）和越来越多的研究人员骨干， UNM HSC，全州和区域性地在他们的研究中实施自噬测定。支持AIM 这些集中的ASC资源将加速mPI向独立NIH的发展轨迹， 因此，R 01级资金避开了mPI通常面临的陡峭的学习曲线。ASC将分享一个新的 改造空间-科学核心实验室（SCICORE实验室）-并将与AIM炎症和 代谢核心。对于不同的应用，两个科学核心将共享AMNIS ImageStream X Mark II流式细胞仪，并将共同参与其升级，应用，开发和监督， 一个技术支持人员的专业知识。ASC还将维持一个自噬转基因动物群体 基因分型和表型质量控制专家。ASC不会重复现有的共享设备 并将与UNM HSC的现有设施以及其他机构的现有核心充分对接 发展奖（IDEA）中心在新墨西哥州。此外，ASC将促进多学科的互动 在UNM HSC和区域之间建立和初级研究人员之间，通过支持广泛的 一系列与自噬有关的基本过程和疾病状态。以下具体目标将是 完成： 具体目标1。创建独特的单一资源来提供专业知识和实验能力， 自噬技术与研究 具体目标2。启用和加强指导PI的自噬研究，并开发新的 在炎症和代谢的背景下研究自噬。 具体目标3。领导，协调，并在整个UNM HSC和国家发展自噬研究。 实现这些目标将有助于R 01达到目前的主要业绩指标名册; 研究人员使用自噬措施;建立一个长期可持续的核心，使AIM研究;和 支持研究在UNM HSC，全州范围内，并在区域IDEA国家。ASC，以及扩展的AIM，将 通过技术讲习班和其他传播方式成为国家资源。