BACTERIAL GENOME RESTRICTION DIGESTS BY FLOW CYTOMETRIC DNA FRAGMENT SIZING

通过流式细胞术 DNA 片段大小测定细菌基因组限制性消化

• 批准号: 7724225
• 负责人: BABETTA L MARRONE
• 金额: $ 3.22万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724225
• 关键词: Bacterial Genome; Computer Retrieval of Information on Scientific Projects Database; DNA; Escherichia coli; Fingerprint; Flow Cytometry; Funding; Grant; Institution; Research; Research Personnel; Resources; Restriction Mapping; Source; Staphylococcus aureus; United States National Institutes of Health; base; size

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DNA fragment size analysis by flow cytometry has been applied to the analysis of restriction enzyme digest of several bacterial strains of E. coli and S. aureus. Distinct restriction fragment fingerprints were observed and served as the basis for discriminating among the strains of each species.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 流式细胞术的 DNA 片段大小分析已应用于大肠杆菌和金黄色葡萄球菌的几种细菌菌株的限制酶消化分析。 观察到独特的限制性片段指纹并作为区分每个物种的菌株的基础。