BACTERIAL GENOME RESTRICTION DIGESTS BY FLOW CYTOMETRIC DNA FRAGMENT SIZING

通过流式细胞术 DNA 片段大小测定细菌基因组限制性消化

• 批准号: 7724225
• 负责人: BABETTA L MARRONE
• 金额: $ 3.22万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724225
• 关键词: Bacterial Genome; Computer Retrieval of Information on Scientific Projects Database; DNA; Escherichia coli; Fingerprint; Flow Cytometry; Funding; Grant; Institution; Research; Research Personnel; Resources; Restriction Mapping; Source; Staphylococcus aureus; United States National Institutes of Health; base; size

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DNA fragment size analysis by flow cytometry has been applied to the analysis of restriction enzyme digest of several bacterial strains of E. coli and S. aureus. Distinct restriction fragment fingerprints were observed and served as the basis for discriminating among the strains of each species.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 用流式细胞仪进行DNA片段大小分析，对几株大肠杆菌和金黄色葡萄球菌的限制性内切酶进行了分析。观察到明显的限制性片段指纹，并作为区分每个物种的菌株的基础。