BACTERIAL GENOME RESTRICTION DIGESTS BY FLOW CYTOMETRIC DNA FRAGMENT SIZING

通过流式细胞术 DNA 片段大小测定细菌基因组限制性消化

• 批准号: 8169373
• 负责人: BABETTA L MARRONE
• 金额: $ 3.34万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169373
• 关键词: Bacterial Genome; Computer Retrieval of Information on Scientific Projects Database; DNA; Escherichia coli; Fingerprint; Flow Cytometry; Funding; Grant; Institution; Research; Research Personnel; Resources; Restriction Mapping; Source; Staphylococcus aureus; United States National Institutes of Health; base

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DNA fragment size analysis by flow cytometry has been applied to the analysis of restriction enzyme digest of several bacterial strains of E. coli and S. aureus. Distinct restriction fragment fingerprints were observed and served as the basis for discriminating among the strains of each species.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 应用流式细胞术对几株大肠杆菌的限制性酶切产物进行了DNA片段大小分析。coli和革兰氏阳性菌S.金黄色。 观察到不同的限制性片段指纹图谱，并作为区分每个种菌株的依据。