DNA FRAGMENT SIZING BY FLOW CYTOMETRY

流式细胞术技术测定DNA的单个片段的大小， 分析其他小的生物粒子正在开发中。 在过去的一年里，人们发现所使用的染料具有 小的动态范围，需要合理准确的DNA知识 染色前的浓度。 一种新的染料，皮科绿色， 发现具有宽得多的动态范围， 在分析之前准确地知道DNA浓度。 电流 努力的重点是将这项技术应用于细菌物种 和菌株鉴定。 详情载于项目3 细菌指纹技术研究进展报告 流式细胞术细菌种属鉴别。

Flow cytometry techniques to size individual fragments of DNA and analyze other small biological particles are under development. During the past year, it was discovered that the dye being used has a small dynamic range requiring reasonably accurate knowledge of DNA concentrations prior to staining. A new dye, Pico Green has been found to have a much wider dynamic range alleviating the need to precisely know the DNA concentration prior to analysis. Current efforts are focused on applying the technology to bacterial species and strain identification. Details are contained in the Project 3 progress report and Research Highlight Bacterial Fingerprinting by Flow Cytometry Bacterial Species Discrimination.