BACTERIAL GENOME RESTRICTION DIGESTS BY FLOW CYTOMETRIC DNA FRAGMENT SIZING

通过流式细胞术 DNA 片段大小测定细菌基因组限制性消化

• 批准号: 8361737
• 负责人: BABETTA L MARRONE
• 金额: $ 2.24万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361737
• 关键词: Bacterial Genome; DNA; Escherichia coli; Fingerprint; Flow Cytometry; Funding; Grant; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Restriction Mapping; Sorting - Cell Movement; Source; Staphylococcus aureus; United States National Institutes of Health; base; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA fragment size analysis by flow cytometry has been applied to the analysis of restriction enzyme digest of several bacterial strains of E. coli and S. aureus. Distinct restriction fragment fingerprints were observed and served as the basis for discriminating among the strains of each species.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 应用流式细胞术对几株大肠杆菌的限制性酶切产物进行了DNA片段大小分析。coli和革兰氏阳性菌S.金黄色。 观察到不同的限制性片段指纹图谱，并作为区分每个种菌株的依据。