STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

真核转录机器的结构研究

• 批准号: 7722108
• 负责人: David A. Bushnell
• 金额: $ 0.06万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722108
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Polymerase II; Development; Elongation Factor; Enzymes; Eukaryota; Eukaryotic Cell; Funding; Gene Expression; Genetic Transcription; Grant; Institution; Mediator of activation protein; Messenger RNA; Mutation Analysis; Pathway interactions; Process; Proteins; RNA Polymerase II; Regulation; Research; Research Personnel; Resources; Source; Transcriptional Regulation; United States National Institutes of Health; pol genes; polypeptide; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 转录是基因表达途径的第一步，也是关键的控制点。转录调控是发育、肿瘤发生和其他基本过程的基础。在真核生物中，核糖核酸聚合酶II(PolI)负责信使RNA的转录，使Polo？S调控成为基因表达的中心。在本提案期间，我们将继续对刑警组织转录机制的结构进行研究。这些研究目前正沿着三个途径进行：第一，使用突变分析详细分析延长的PolII；第二，PolII与起始和延长因子的共晶；第三，对介体复合体的结构研究，这是一种POL对调节蛋白做出反应所需的22个多肽复合体。