STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

真核转录机器的结构研究

• 批准号: 7722108
• 负责人: David A. Bushnell
• 金额: $ 0.06万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722108
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Polymerase II; Development; Elongation Factor; Enzymes; Eukaryota; Eukaryotic Cell; Funding; Gene Expression; Genetic Transcription; Grant; Institution; Mediator of activation protein; Messenger RNA; Mutation Analysis; Pathway interactions; Process; Proteins; RNA Polymerase II; Regulation; Research; Research Personnel; Resources; Source; Transcriptional Regulation; United States National Institutes of Health; pol genes; polypeptide; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.

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