STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

真核转录机器的结构研究

• 批准号: 7722108
• 负责人: David A. Bushnell
• 金额: $ 0.06万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722108
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Polymerase II; Development; Elongation Factor; Enzymes; Eukaryota; Eukaryotic Cell; Funding; Gene Expression; Genetic Transcription; Grant; Institution; Mediator of activation protein; Messenger RNA; Mutation Analysis; Pathway interactions; Process; Proteins; RNA Polymerase II; Regulation; Research; Research Personnel; Resources; Source; Transcriptional Regulation; United States National Institutes of Health; pol genes; polypeptide; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 转录是基因表达途径的第一步，也是关键控制点。转录调控是发育、肿瘤发生和其他基本过程的基础。在真核生物中，RNA 聚合酶 II (pol) 负责信使 RNA 的转录，使 pol 的调节成为基因表达的核心。在本提案期间，我们将继续对 pol 转录机制进行结构研究。这些研究目前沿着三个途径进行：首先，使用突变分析对延长的pol II进行详细分析；第二，具有引发和延伸因子的 pol II 共晶；第三，介导复合物的结构研究，介导复合物是 pol 响应调节蛋白所需的 22 种多肽复合物。