STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

真核转录机器的结构研究

• 批准号: 8362149
• 负责人: David A. Bushnell
• 金额: $ 1.56万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362149
• 关键词: Complex; DNA Polymerase II; Development; Elongation Factor; Enzymes; Eukaryota; Funding; Gene Expression; Genetic Transcription; Grant; Mediator of activation protein; Messenger RNA; Mutation Analysis; National Center for Research Resources; Pathway interactions; Peptide Initiation Factors; Principal Investigator; Process; Proteins; RNA Polymerase II; Radiation; Regulation; Research; Research Infrastructure; Resources; Source; Transcriptional Regulation; United States National Institutes of Health; cost; polypeptide; structural biology; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 转录是基因表达途径的第一步，也是基因表达的关键控制点。转录调控是发育、肿瘤发生和其他基本过程的基础。在真核生物中，RNA聚合酶II（pol）负责信使RNA的转录，使pol？的调控中心的基因表达。在此期间的建议，我们将继续我们的结构研究的pol转录机制。这些研究目前正在进行沿着三个途径：第一，详细分析的延伸polII使用突变分析;第二，共晶体的polII与起始和延伸因子;第三，结构研究的调解人复杂的，一个22多肽复合物所需的pol响应调节蛋白质。