INITIAL SOLUTION X-RAY SCATTERING STUDIES ON RNA POLYMERASE II-TBP-TFIIB-TFIIF-P

RNA 聚合酶 II-TBP-TFIIB-TFIIF-P 初始溶液 X 射线散射研究

• 批准号: 7722076
• 负责人: David A. Bushnell
• 金额: $ 0.08万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722076
• 关键词: Biochemical; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Polymerase II; Enzymes; Eukaryotic Cell; Funding; Gene Expression; General Transcription Factors; Genetic Transcription; Grant; Institution; Modeling; Numbers; Pathway interactions; Polymerase; Process; RNA Polymerase II; Research; Research Personnel; Resolution; Resources; Solutions; Source; Structure; Transcription Factor TFIIB; Transcription Initiation; United States National Institutes of Health; human GTF2B protein; insight; promoter; transcription factor TFIIF

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step and the key control point in the pathway of gene expression. The central enzyme in transcription for gene expression in eukaryotic cells is RNA polymerase II (pol II), which requires a number of accessory factors, referred to as general transcription factors (TF) for recognizing a promoter and specifically initiating transcription. Pol II serves as a platform for the assembly of transcriptional initiation and elongation complexes, through its interactions with the general transcription factors. The near atomic- resolution structure of the general transcription factor TFIIB co- crystallized with pol II at near atomic-resolution has given unexpected insight into the mechanism of the transcription initiation process. We have also studied the transcriptional initiation complexes formed pol II with TBP, TFIIB, TFIIF, and promoter DNA. Combining the structural information as well as biochemical evidence we propose a model for what the basal transcription initiation complex may look like, however we have so far no direct structural evidence to confirm this model.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 转录是基因表达途径的第一步，也是关键的控制点。真核细胞基因表达的核心转录酶是RNA聚合酶II(PolII)，它需要一系列的辅助因子，被称为通用转录因子(Tf)，用于识别启动子并特异性地启动转录。POL II通过与一般转录因子的相互作用，为转录起始和延伸复合体的组装提供了平台。通用转录因子TFIIB与PolII在近原子分辨率下共结晶的近原子分辨率结构为转录起始过程的机制提供了意想不到的见解。我们还研究了PolII与TBP、TFIIB、TFIIF和启动子DNA形成的转录起始复合体。结合结构信息以及生化证据，我们提出了一个基本转录起始复合体可能是什么样子的模型，但到目前为止，我们还没有直接的结构证据来证实这一模型。