TANDEM QUADRUPOLE MASS SPECTROMETRY OF SULFATIDES

硫化物的串联四极杆质谱法

• 批准号: 7721460
• 负责人: FONG-FU HSU
• 金额: $ 0.89万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721460
• 关键词: Class; Computer Retrieval of Information on Scientific Projects Database; Electrospray Ionization; Fatty Acids; Funding; Grant; Hand; Institution; Ions; Location; Mass Spectrum Analysis; Molecular; Process; Research; Research Personnel; Resources; Scanning; Source; Staging; Structure; Sulfoglycosphingolipids; United States National Institutes of Health; analog; base; hydroxy fatty acid; mass spectrometer; tandem mass spectrometry; unsaturated bonds

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sulfatides yield intense deprotonated molecular species ([M - H]-) in negative-ion mode by electrospray ionization. The product-ion spectra of the [M - H]- ions of sulfatides obtained with a tandem quadrupole and with an ion-trap mass spectrometers contain abundant fragment ions informative for structural determination of the long-chain base and the fatty acid constituent including the location of its unsaturated bond. Identifications of sulfatides in mixtures, or specific sulfatide subclasses that are varied by long-chain base, or differentiation of sulfatide class with a-hydroxy fatty acid substituent from that with non-hydroxy fatty acid group can be facilitated by precursor ion or constant neutral loss scans. While CAD product-ion spectra of the [M - H]- ions are useful for differentiation of isomeric structures, the mechanisms underlying the fragmentation processes for sulfatides under low-energy CAD can be unveiled by multiple-stage ion-trap tandem mass spectrometry of various sulfatides, including their H-D exchanged analogs. The sensitivity of sulfatides observed as the [M + Na]+ ions by ESI in the positive-ion mode, on the other hand, is poor. The product-ion spectra of the [M + Na]+ ions of sulfatides contain very few structurally informative ions and are not useful for structural identification.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 通过电喷雾电离，硫脂在负离子模式下产生强烈的去质子化分子种类 ([M - H]-)。使用串联四极杆和离子阱质谱仪获得的硫苷脂的 [M - H]- 离子的产物离子谱包含丰富的碎片离子信息，可用于确定长链碱基和脂肪酸成分（包括其不饱和键的位置）的结构。通过前体离子或恒定中性丢失扫描，可以促进混合物中硫苷脂的鉴定，或因长链碱基而异的特定硫苷脂亚类，或具有α-羟基脂肪酸取代基的硫苷脂类与具有非羟基脂肪酸基团的硫苷脂类的区分。虽然 [M - H]- 离子的 CAD 产物离子谱可用于区分异构体结构，但低能 CAD 下硫苷脂碎裂过程的机制可以通过各种硫苷脂（包括其 H-D 交换类似物）的多级离子阱串联质谱法来揭示。另一方面，ESI 在正离子模式下以 [M + Na]+ 离子形式观察到的脑硫苷脂的灵敏度较差。硫苷脂的 [M + Na]+ 离子的产物离子谱包含很少的结构信息离子，对于结构鉴定没有用处。