STABLE ISOTOPE LABELING METHOD DEVELOPMENT TO MEASURE PROTEIN TURNOVER IN A MUL

稳定同位素标记方法的开发用于测量 MUL 中的蛋白质周转率

• 批准号: 7723727
• 负责人: Michael MacCoss
• 金额: $ 1.73万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723727
• 关键词: Adult; Animals; Caenorhabditis elegans; Computer Retrieval of Information on Scientific Projects Database; Development; Development, Other; Funding; Grant; IGF-1 Signaling Pathway; Institution; Insulin; Life; Ligands; Longevity; Mass Spectrum Analysis; Measures; Metabolism; Methodology; Organism; Pathway interactions; Process; Production; Protein Overexpression; Proteins; Research; Research Personnel; Resources; Source; Stable Isotope Labeling; Staging; United States National Institutes of Health; improved; insulin signaling; interest; method development; protein degradation; research study; time interval

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are conducting experiments using stable isotope labeling and mass spectrometry to measure protein turnover in C. elegans. The turnover of protein is measured by comparing the incorporation of 15N into proteins over different time intervals. These experiments are being conducted with the adult glp-4 strain in the background of all strains to suppress worm progeny production and therefore keep all animals at the same life stage. This methodology can be used to look at the turnover of proteins in multiple organisms during development and other processes. We specifically are interested in measuring turnover of proteins in the insulin/IGF-1 signaling pathway in C. elegans while overexpressing the ligands of the pathway. Proteins in the insulin/IGF-1 signaling pathway have been shown to be involved in longevity, metabolism and development. By looking at the turnover of proteins in this pathway during ligand overexpression, we hope to improve our understanding of the mechanisms relating to insulin signaling.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们正在使用稳定同位素标记和质谱法进行实验来测量线虫中的蛋白质周转率。 通过比较不同时间间隔内 15N 掺入蛋白质的情况来测量蛋白质的周转率。 这些实验是在所有菌株的背景下以成体glp-4菌株进行的，以抑制蠕虫后代的产生，从而使所有动物保持在同一生命阶段。 这种方法可用于观察多种生物体在发育和其他过程中蛋白质的周转。 我们特别感兴趣的是测量线虫中胰岛素/IGF-1信号通路中蛋白质的周转，同时过度表达该通路的配体。 胰岛素/IGF-1 信号通路中的蛋白质已被证明与寿命、新陈代谢和发育有关。 通过观察配体过度表达过程中该途径中蛋白质的周转，我们希望增进对胰岛素信号转导相关机制的理解。