Modelling stem cell decisions in mouse germ cells

模拟小鼠生殖细胞中的干细胞决策

• 批准号: DP140104059
• 负责人: A/Prof Josephine Bowles
• 金额: $ 26.89万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140104059
• 关键词: Modelling; stem; cell; decisions; mouse

A fundamental problem in biology is how cells transition from pluripotency to lineage commitment. The aim of this project is to study this problem, in vivo, by dissecting the mechanisms active during normal development of primordial germ cells (PGCs, gamete stem cells) in the mouse fetal testis. Using molecular, cellular and whole animal assays three central hypotheses will be addressed regarding genes likely to be important in the process. This research will reveal the molecular nature of bipotential switches in cell identity and will inform some of the most important biological issues of our time, including tissue regeneration, aging and cancer biology.

生物学的一个基本问题是细胞如何从多能性转变为谱系承诺。该项目的目的是通过解剖小鼠胎儿睾丸中原始生殖细胞（PGC，配子干细胞）的正常发育过程中活跃的机制来研究这个问题。使用分子，细胞和整个动物测定，将解决有关此过程中可能很重要的基因的三个中心假设。这项研究将揭示细胞同一性中双性转换的分子性质，并将告知我们这个时代最重要的生物学问题，包括组织再生，衰老和癌症生物学。