UNCOVERING HIDDEN IN VIVO RESONANCES USING EDITING BASED

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 提出了一种用于体内质子NMR的新型单次激发光谱编辑技术，以恢复被非常强的共振掩盖的低浓度代谢物的共振。利用这种新方法，编辑是通过使用绝热脉冲的hommandelHartmann-Hahn极化转移将横向磁化转移到来自所选耦合伙伴的J耦合自旋来进行的。目前的实施方案使用基于激光的具有单体素定位的1D-TOCSY，以从水下恢复4.63 ppm的β-葡萄糖的H1质子，并从强脂质信号下方恢复乳酸甲酯共振。该方法可以扩展到传统编辑方法难以执行的其他旋转系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel single-shot spectral editing technique for in vivo proton NMR is proposed to recover resonances of low-concentration metabolites obscured by very strong resonances. With this new method, editing is performed by transferring transverse magnetization to J-coupled spins from selected coupling partners using a homonuclear Hartmann-Hahn polarization transfer with adiabatic pulses. The current implementation uses 1D-TOCSY with single-voxel localization based on LASER to recover the H1 proton of beta-glucose at 4.63 ppm from under water and the lactate methyl resonances from beneath a strong lipid signal. The method can be extended to further spin systems where conventional editing methods are difficult to perform.