UNCOVERING HIDDEN IN VIVO RESONANCES USING EDITING BASED

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 提出了一种新的体内质子核磁共振单次光谱编辑技术，以恢复被非常强的共振遮挡的低浓度代谢物的共振。在这种新的方法中，编辑是通过利用绝热脉冲的同核Hartmann-Hahn极化转移将横向磁化转移到J耦合自旋来实现的。目前的实现使用基于激光的单体素定位的1D-TOCSY从水下恢复4.63ppm的β-葡萄糖的H1质子，并从强脂信号下恢复乳酸甲基共振。该方法可以扩展到常规编辑方法难以执行的进一步旋转系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel single-shot spectral editing technique for in vivo proton NMR is proposed to recover resonances of low-concentration metabolites obscured by very strong resonances. With this new method, editing is performed by transferring transverse magnetization to J-coupled spins from selected coupling partners using a homonuclear Hartmann-Hahn polarization transfer with adiabatic pulses. The current implementation uses 1D-TOCSY with single-voxel localization based on LASER to recover the H1 proton of beta-glucose at 4.63 ppm from under water and the lactate methyl resonances from beneath a strong lipid signal. The method can be extended to further spin systems where conventional editing methods are difficult to perform.