UNCOVERING HIDDEN IN VIVO RESONANCES USING EDITING BASED

使用基于编辑的方法揭示隐藏的体内共振

• 批准号: 7601632
• 负责人: MALGORZATA MARJANSKA
• 金额: $ 2.36万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601632
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Coupling; Funding; Glucose; Grant; Institution; Lasers; Lipids; Methods; Physiologic pulse; Protons; Pulse taking; Research; Research Personnel; Resources; Signal Transduction; Source; System; Techniques; United States National Institutes of Health; Water; base; in vivo; methyl lactate; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A novel single-shot spectral editing technique for in vivo proton NMR is proposed to recover resonances of low-concentration metabolites obscured by very strong resonances. With this new method, editing is performed by transferring transverse magnetization to J-coupled spins from selected coupling partners using a homonuclear Hartmann-Hahn polarization transfer with adiabatic pulses. The current implementation uses 1D-TOCSY with single-voxel localization based on LASER to recover the H1 proton of beta-glucose at 4.63 ppm from under water and the lactate methyl resonances from beneath a strong lipid signal. The method can be extended to further spin systems where conventional editing methods are difficult to perform.

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