PROLONGED EXPOSURE TO DIOXANE & ACETONITRILE OF SPIN-LABEL SUBTILISIN CARSBERG

长期接触二恶烷

• 批准号: 7724010
• 负责人: DMITRIY TIPIKIN
• 金额: $ 1.59万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724010
• 关键词: Acetonitriles; Active Sites; Admixture; Computer Retrieval of Information on Scientific Projects Database; Dioxanes; Exposure to; Funding; Grant; Helium; Institution; Knowledge; Laboratories; Nitrogen; Phase; Positioning Attribute; Research; Research Personnel; Resources; Sampling; Source; Spin Labels; Subtilisin; Subtilisins; Temperature; United States National Institutes of Health; Width; molecular dynamics; professor; research study; water solution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sample of subtilisin Carsberg spin labeled at an active site was prepared in the laboratory of Professor Gabriel Barletta and was sent to ACERT to be investigated by high-field EPR. The idea was to determine accurately the parameters of the g-tensor to use it later at Professor Barletta group for simulations of molecular dynamic at X-band. The sample was transferred into the resonator and it was cooled with either nitrogen or helium. The experiments were done at 190 K for dioxane, and, since there is not a real rigid limit at this temperature, also at 80 K to guarantee the rigid limit. Modulation used was 5 G. After the experiments some of the spectra were phased to get rid of a small admixture of dispersion. For some of the spectra a small passage effect was observed, but this effect does not change the position of the lines. The g-factor was determined by comparison with the known g-factor of PDTempone. The sweep width was determined from the knowledge of the separation between the lines for water solution of Mn.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在Gabriel Barletta教授的实验室准备了在活跃部位标记的枯草菌素CARSBERG自旋样品，并被送往Acert，由高田EPR进行研究。这个想法是在Barletta组教授组中准确确定G量的参数，以模拟X波段的分子动力学。将样品转移到谐振器中，并用氮或氦气冷却。实验是在190 k的二恶英完成的，并且由于在该温度下没有真正的刚性极限，因此在80 K处也可以保证刚性极限。使用的调节为5 g。实验后，一些光谱被逐步分析以消除少量分散体。对于某些光谱，观察到小传递效应，但是这种效果不会改变线的位置。通过与已知的pDtempone的G因子进行比较确定G因子。扫描宽度是从Mn水溶液线之间的分离的知识中确定的。