PROLONGED EXPOSURE TO DIOXANE & ACETONITRILE OF SPIN-LABEL SUBTILISIN CARSBERG

长期接触二恶烷

• 批准号: 7724010
• 负责人: DMITRIY TIPIKIN
• 金额: $ 1.59万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724010
• 关键词: Acetonitriles; Active Sites; Admixture; Computer Retrieval of Information on Scientific Projects Database; Dioxanes; Exposure to; Funding; Grant; Helium; Institution; Knowledge; Laboratories; Nitrogen; Phase; Positioning Attribute; Research; Research Personnel; Resources; Sampling; Source; Spin Labels; Subtilisin; Subtilisins; Temperature; United States National Institutes of Health; Width; molecular dynamics; professor; research study; water solution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The sample of subtilisin Carsberg spin labeled at an active site was prepared in the laboratory of Professor Gabriel Barletta and was sent to ACERT to be investigated by high-field EPR. The idea was to determine accurately the parameters of the g-tensor to use it later at Professor Barletta group for simulations of molecular dynamic at X-band. The sample was transferred into the resonator and it was cooled with either nitrogen or helium. The experiments were done at 190 K for dioxane, and, since there is not a real rigid limit at this temperature, also at 80 K to guarantee the rigid limit. Modulation used was 5 G. After the experiments some of the spectra were phased to get rid of a small admixture of dispersion. For some of the spectra a small passage effect was observed, but this effect does not change the position of the lines. The g-factor was determined by comparison with the known g-factor of PDTempone. The sweep width was determined from the knowledge of the separation between the lines for water solution of Mn.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 加布里埃尔·巴莱塔教授的实验室制备了活性部位标记的枯草杆菌毒素Carsberg自旋的样品，并将其送到Acert进行高场EPR研究。这个想法是为了准确地确定g张量的参数，以便稍后在Barletta教授小组用于模拟X带的分子动力学。样品被转移到谐振器中，并用氮气或氦冷却。实验是在190K对二恶烷进行的，由于在这个温度下没有真正的刚性极限，所以也在80K来保证刚性极限。使用的调制是5G。在实验之后，一些光谱被分相以去除少量的色散混合物。对于一些光谱，观察到了一个小的通过效应，但这种效应并不改变谱线的位置。通过与已知的PDTempone的g因子进行比较，确定了g因子。根据对水溶液中锰的谱线间距的了解，确定了扫描宽度。