EPR INVESTIGATIONS OF IRON SIDEROPHORE CHELATES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 铁载体是结合铁并增加细菌铁转运速率的细菌产物。还已知一些铁结合化合物在含有动物或人血清的培养基中起作用。 由于铁螯合是一种已知的刺激细菌生长感染期间，铁载体的合成已被链接到细菌的毒力。 铜绿假单胞菌在铁限制条件下产生两种具有不同螯合性质的铁载体，绿脓菌荧光素（PVD）和绿脓菌螯铁蛋白（PCH）。 为了阐明的性质的铁铁载体配体中的两个P. Aerobium铁载体，我们正在进行低温9.5 GHz的EPR研究，这是特别敏感的铁配体结构的细节。 该服务项目补充了希望使用中心资源进行铁-蛋白质相互作用研究的其他中心用户的请求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Siderophores are bacterial products which bind iron and increase the rate of bacterial iron transport. Some iron-binding compounds are also known to function in media containing animal or human serum. Since iron sequestration is a known stimulus of bacterial growth during infections, siderophore synthesis has been linked to bacterial virulence. Pseudomonas aeruginosa produces two siderophores under iron-limiting conditions with different chelating properties, pyoverdine (PVD) and pyochelin (PCH). In order to elucidate the nature of the iron siderophore ligands in the two P. Aeruginosa siderophores, we are undertaking low temperature 9.5 GHz EPR studies which are particularly sensitive to the details of the iron ligand structure. This service project complements requests from other center users who wish to use center resources for studies of iron-protein interactions.