A Multi-functional Optical Impedance Microscope for Live Cell Imaging

用于活细胞成像的多功能光阻抗显微镜

• 批准号: 7762888
• 负责人: SHAOPENG WANG
• 金额: $ 17.83万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-15 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7762888
• 关键词: Address; Algorithms; Behavior; Biological; Biological Assay; Biological Models; Biomedical Research; Biosensor; Cell Adhesion; Cell Culture Techniques; Cell Extracts; Cell surface; Cell-Matrix Junction; Cells; Cellular Morphology; Charge; Chemicals; Clinical; Confocal Microscopy; Dependence; Development; Electric Capacitance; Electrodes; Event; Figs - dietary; Film; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Glass; Goals; Gold; Growth; Image; Image Analysis; Imaging Techniques; In Situ; Individual; Label; Lateral; Life; Light; Maps; Measurement; Measures; Mechanics; Methodology; Methods; Microfabrication; Microscope; Microscopy; Modeling; Monitor; Noise; Optics; Phase; Phase-Contrast Microscopy; Process; Research Personnel; Resolution; Sampling; Shunt Device; Signal Transduction; Slide; Spectrum Analysis; Substrate Interaction; Surface; Surface Plasmon Resonance; System; Techniques; Technology; Testing; Time; Toxicology; Work; Wound Healing; angiogenesis; base; cell behavior; cell motility; cellular imaging; data acquisition; density; electric impedance; image processing; imaging modality; innovation; instrument; interest; novel; public health relevance; research study; sensor; submicron; success; tool; voltage

DESCRIPTION (provided by applicant): Project Summary Electrical impedance spectroscopy (EIS) is a sensitive label-free technique that has proved to be a powerful tool for a wide range of live cell studies. Measurement of the local impedance of live cells on an entire surface is highly desired, but so far has not been possible with current EIS technology. This project aims at the development of a new microscopy that can capture high-resolution impedance images of live cells. The proposed new microscopy is based on principles that are completely different from the conventional EIS. Instead of measuring impedance electrically, it images the local impedance of the entire surface optically with sub-micron spatial resolution. This simplifies the impedance measurement without sacrificing sensitivity and, more importantly, it introduces new exciting capabilities including: 1) sensor chips can be easily fabricated and prepared for cell attachment; 2) the entire sensor chip or selected region of interest can be analyzed for detailed studies, which is important because it enables the tracking of individual cells or even region within single cells with the best sensitivity and spatial resolution; 3) conventional surface plasmon resonance images can be obtained simultaneously, which provide detailed information on cell/substrate interaction; and 4) the instrument will be built based on a conventional inverted optical microscope, so that in-situ phase contrast and fluorescence microscopy images can be obtained for the same sample if desired. The project includes the following four tasks: 1) build a high-resolution optical impedance microscope system; 2) establish data acquisition, processing, and analysis algorithms for live cell analysis; 3) study the relationships between impedance microscopy images and cell adhesion behavior; and 4) test and evaluate the optical impedance microscope for additional studies of cells including wound healing, toxicology and motility. PUBLIC HEALTH RELEVANCE (provided by applicant): This project aims at the development of a new label-free microscopy that can capture sub-micron resolution impedance images of live cells optically. In addition, conventional surface plasmon resonance, optical and fluorescence microscopy images can be obtained simultaneously. The success of this project will provide a new tool that has a broad range of applications on cell dynamic studies.

描述（由申请人提供）：项目概述电阻抗谱（EIS）是一种灵敏的无标记技术，已被证明是广泛的活细胞研究的有力工具。非常需要测量整个表面上活细胞的局部阻抗，但目前的EIS技术还不可能。该项目旨在开发一种新的显微镜，可以捕获活细胞的高分辨率阻抗图像。提出的新显微镜是基于完全不同于传统的EIS的原则。它不是以电学方式测量阻抗，而是以亚微米空间分辨率光学成像整个表面的局部阻抗。这简化了阻抗测量而不牺牲灵敏度，更重要的是，它引入了新的激发能力，包括：1）传感器芯片可以容易地制造和准备用于细胞附着; 2）可以分析整个传感器芯片或所选择的感兴趣区域以进行详细研究，这是重要的，因为它能够以最佳灵敏度和空间分辨率跟踪单个细胞或甚至单个细胞内的区域; 3）可以同时获得常规的表面等离子体共振图像，其提供关于细胞/基质相互作用的详细信息;以及4）该仪器将基于常规的倒置光学显微镜来构建，使得如果需要，可以获得相同样品的原位相差和荧光显微镜图像。该项目包括以下四个任务：1）建立一个高分辨率的光学阻抗显微镜系统; 2）建立活细胞分析的数据采集，处理和分析算法; 3）研究阻抗显微镜图像和细胞粘附行为之间的关系;和4）测试和评估光学阻抗显微镜用于细胞的其他研究，包括伤口愈合，毒理学和运动。 公共卫生相关性（由申请人提供）：该项目旨在开发一种新的无标记显微镜，可以光学捕获活细胞的亚微米分辨率阻抗图像。此外，传统的表面等离子体共振，光学和荧光显微镜图像可以同时获得。该项目的成功将为细胞动力学研究提供一种新的工具，具有广泛的应用。