A cost-effective bioreactor to advance functional tissue engineering of cartilage

一种具有成本效益的生物反应器，可推进软骨功能组织工程

• 批准号: 7908519
• 负责人: Trevor Justin Lujan
• 金额: $ 10.02万
• 依托单位: APEX BIOMEDICAL COMPANY, LLC
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7908519
• 关键词: Achievement; Amplifiers; Biochemistry; Biomechanics; Bioreactors; Cartilage; Chemicals; Chronic; Clinical; Communication; Connective Tissue; Degenerative polyarthritis; Development; Devices; Disease; Electromagnetics; Engineering; Europe; Evaluation; Fibrocartilages; Fostering; Goals; Growth; Individual; Laboratories; Maps; Marketing; Materials Testing; Measurement; Measures; Mechanical Stimulation; Mechanics; Methodology; Methods; Modeling; Morphology; Partner in relationship; Patient Care; Performance; Phase; Prevention; Property; Protocols documentation; Research; Small Business Technology Transfer Research; Software Validation; Specimen; Speed; Staging; Stimulus; System; Techniques; Technology; Technology Transfer; Testing; Tissue Engineering; Tissues; Translations; United States; Validation; articular cartilage; bone; cost; design; design and construction; disability; functional outcomes; graphical user interface; improved; in vivo; innovation; meetings; operation; public health relevance; regenerative; research study; scaffold; scale up; sensor; soft tissue; treatment strategy

DESCRIPTION (provided by applicant): Osteoarthritis (OA) is the leading cause of chronic disability in the United States. A clinical goal in the treatment and prevention of OA is to develop replacement cartilage using tissue engineering (TE) technologies. Although TE cartilage presently lacks the mechanical stability of native cartilage, studies have demonstrated that mechanical stability can be enhanced with specific chemical and mechanical stimuli. To speed the discovery of optimal stimulation protocols, research platforms need to be available that enable fast, clear and reliable communication of functional outcomes (i.e material properties). Towards this goal, we introduce a six-chamber bioreactor that combines the efficiency of batch testing with the accuracy normally reserved for dedicated single-specimen material test systems. This system is therefore capable of mapping functional development of six individual specimens exposed to highly-specific mechanical stimulation protocols. To remain cost-effective and portable, the bioreactor leverages system redundancies to eliminate hardware. The specific aim of this study is to test the bioreactor's capacity to deliver accurate mechanical stimulations and material property evaluations in all six test chambers. The effect of loading conditions and specimen geometry on accurate mechanical stimulation will be quantified using external sensors. The viscoelastic material properties of soft TE scaffolds and stiff cartilage plugs will be characterized in both the six-chamber bioreactor and a conventional single-stage testing device. Results between the bioreactor and the model testing system will be statistically compared. If validation of the bioreactor is successful, we envision this product will provide an economical and reliable research platform that fosters TE technology transfer. PUBLIC HEALTH RELEVANCE: Tissue engineering of articular cartilage presents a promising strategy for treatment of osteoarthritis, a debilitating and prevalent disease. Cartilage engineering techniques, however, are currently unable to reproduce the mechanical properties critical to native cartilage, thus impeding the transfer of TE technology to patient care. A bioreactor is therefore proposed to facilitate the rapid discovery of mechanical conditions that promote the synthesis of mechanically viable tissue.

描述（由申请人提供）：骨关节炎 (OA) 是美国慢性残疾的主要原因。治疗和预防 OA 的临床目标是利用组织工程 (TE) 技术开发替代软骨。尽管 TE 软骨目前缺乏天然软骨的机械稳定性，但研究表明，可以通过特定的化学和机械刺激来增强机械稳定性。为了加速发现最佳刺激方案，需要提供能够快速、清晰和可靠地传达功能结果（即材料特性）的研究平台。为了实现这一目标，我们引入了一种六室生物反应器，它将批量测试的效率与通常为专用单样本材料测试系统保留的精度结合起来。因此，该系统能够绘制暴露于高度特异性机械刺激方案的六个个体样本的功能发育图。为了保持成本效益和便携性，生物反应器利用系统冗余来消除硬件。这项研究的具体目的是测试生物反应器在所有六个测试室中提供准确的机械刺激和材料特性评估的能力。负载条件和样本几何形状对精确机械刺激的影响将使用外部传感器进行量化。软 TE 支架和硬软骨塞的粘弹性材料特性将在六室生物反应器和传统的单级测试装置中进行表征。生物反应器和模型测试系统之间的结果将进行统计比较。如果生物反应器的验证成功，我们预计该产品将提供一个经济且可靠的研究平台，促进 TE 技术转让。 公共健康相关性：关节软骨的组织工程为治疗骨关节炎（一种使人衰弱的普遍疾病）提供了一种有前景的策略。然而，软骨工程技术目前无法重现对天然软骨至关重要的机械特性，从而阻碍了 TE 技术向患者护理的转移。因此，提出了一种生物反应器来促进快速发现促进机械上可行的组织合成的机械条件。