MicroRNA Expression Profiling in Idiopathic Pulmonary Fibrosis

描述（由申请人提供）： 特发性肺纤维化（IPF）是以肺部炎症和纤维化为特征的慢性间质性肺疾病。IPF的发病机制涉及多个细胞间相互作用、多个信号转导通路的串扰和基因调控。MicroRNA是一种非编码的22个核苷酸的小RNA，与许多生物过程和疾病有关。microRNA调节约1/3的mRNA。然而，关于miRNAs在IPF中的作用尚不清楚。我们推测，在IPF的进展过程中，miRNAs表达失调，这些miRNAs调节导致IPF发展的基因。使用miRNA微阵列，我们将对LTRC IPF样本进行microRNA微阵列分析。将通过实时PCR验证鉴定的miRNA。所选microRNA的细胞定位将通过原位杂交来确定。这项初步研究将为进一步研究miRNAs在IPF中的功能提供基础。公共卫生相关性：该提案的完成将进一步加深我们对IPF发病机制的了解，并为开发新的治疗策略提供基础。(End摘要）

DESCRIPTION (provided by applicant): Idiopathic Pulmonary Fibrosis (IPF) is a chronic interstitial lung diseases characterized by pulmonary inflammation and fibrosis. The pathogenesis of IPF involves multiple cell-cell interactions, cross-talk of multiple signal transduction pathways and gene regulation. MicroRNA is a non-coding ~22-nucleotide small RNA implicated in many biological processes and diseases. MicroRNAs regulate ~ 1/3 of mRNAs. However, nothing is known regarding the role of miRNAs in IPF. We hypothesize that miRNAs are dys-regulated during the progression of IPF and that these miRNAs regulate genes responsible for the development of IPF. Using a miRNA microarray, we will perform microRNA microarray analyses of LTRC IPF samples. The identified miRNAs will be verified by real-time PCR. The cellular localizations of the selected microRNAs will be determined by in situ hybridization. This pilot study will provide the basis for further functional studies of miRNAs in IPF. PUBLIC HEALTH RELEVANCE: The completion of this proposal will further our knowledge about the pathogenesis of IPF as well as provide a baseboard for the development of novel therapeutic strategies. (End of Abstract)