Molecular Biology Core

分子生物学核心

• 批准号: 10459262
• 负责人: LIN LIU
• 金额: $ 24.13万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10459262
• 关键词: Address; Adenovirus Vector; Adopted; Bacteria; Biological Assay; CRISPR/Cas technology; Cell Separation; Cells; Communicable Diseases; Consultations; Deletion Mutagenesis; Development; Funding; Gene Silencing; Genes; Individual; Infectious Diseases Research; Institution; Lentivirus Vector; Lung; Methods; Molecular; Molecular Biology; Mutagenesis; Non-Viral Vector; Oklahoma; Pathway interactions; Phase; Pilot Projects; Production; Pseudomonas aeruginosa; RNA Interference; Reagent; Reporter; Research Personnel; Research Project Grants; Respiration; Respiratory Center; Respiratory Disease; Services; Streptococcus pneumoniae; Technical Expertise; Techniques; Technology; Time; Training; Transfection; Transgenes; base; continuous cell line; design; gain of function; gene transfer vector; genome-wide; influenzavirus; loss of function; overexpression; respiratory pathogen; respiratory virus; transcriptome sequencing; transgene expression

PROJECT SUMMARY All the Phase II CoBRE projects utilize molecular biology approaches to understand the molecular mechanisms of respiratory diseases caused by respiratory pathogens, including influenza virus (Project 1 and Project 2), Streptococcus pneumoniae (Project 3) and Pseudomonas aeruginosa (Project 4). The overall objective of the Molecular Biology Core (MBC) is to provide centralized services and technical expertise in molecular biology for Phase II CoBRE project investigators, pilot project investigators, and other investigators. The specific aims of the MBC are to provide services and support for 1) functional studies using RNAi and CRISPR/Cas9, transgene overexpression, and mutagenesis/deletion, 2) genome-wide gene profiling using RNA sequencing, 3) molecular assays including reporter assays and real-time quantitative PCR, 4) reagents for respiratory and infectious disease research including primary lung cells, respiratory viruses and bacteria, and 5) advice, consultation and training on various aspects of molecular biology and developing or adopting new methods as needed.

项目摘要 所有第二阶段的CoBRE项目都利用分子生物学方法来了解分子生物学。 包括流感病毒在内的呼吸道病原体引起的呼吸道疾病的机制（项目1和 项目2）、肺炎链球菌（项目3）和铜绿假单胞菌（项目4）。整体 分子生物学核心（MBC）的目标是提供集中的服务和技术专长， 为II期CoBRE项目研究者、试点项目研究者和其他研究者提供分子生物学。 MBC的具体目标是为以下方面提供服务和支持：1）使用RNAi的功能研究， CRISPR/Cas9、转基因过表达和诱变/缺失，2）使用CRISPR/Cas9的全基因组基因谱分析， RNA测序，3）分子测定，包括报告基因测定和实时定量PCR，4）试剂 用于呼吸道和传染病研究，包括原代肺细胞、呼吸道病毒和细菌， 和5）分子生物学各方面的建议、咨询和培训，以及开发或采用 新的方法是必要的。