BLUNTED LIPOLYSIS AND FATTY ACID OXIDATION DURING MODERATE EXERCISE IN HIV

HIV 患者适度运动期间脂肪分解和脂肪酸氧化减弱

• 批准号: 7953980
• 负责人: TODD CADE
• 金额: $ 0.64万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953980
• 关键词: Adipose tissue; Blood; Body Composition; Collection; Computer Retrieval of Information on Scientific Projects Database; DEXA; Exercise; Fasting; Fatty acid glycerol esters; Funding; Glucose; Glucose Intolerance; Glycerol; Grant; HIV; HIV Seropositivity; Highly Active Antiretroviral Therapy; Hyperlipidemia; Institution; Insulin; Insulin Resistance; Lipolysis; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measures; Minor; Moderate Exercise; Monitor; OGTT; Palmitates; Plasma; Protease Inhibitor; Recovery; Research; Research Personnel; Resources; Rest; Ritonavir; Source; Tracer; United States National Institutes of Health; biomedical resource; fatty acid oxidation; glucose tolerance; insulin sensitivity; oxidation; regional difference; stable isotope

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The protease inhibitor (PI) ritonavir (RTV) has been associated with elevated resting lipolytic rate, hyperlipidemia, and insulin resistance/glucose intolerance. The purpose of this study was to examine relationships between lipolysis and fatty acid (FA) oxidation during rest, moderate exercise and recovery, and measures of insulin sensitivity/glucose tolerance and fat redistribution in HIV-positive subjects taking RTV (n=12), HAART but no PI (n=10), and HIV-seronegative controls (n=10). Stable isotope tracers [1-(13)C]palmitate and [1,1,2,3,3-(2)H5]glycerol were continuously infused with blood and breath collection during 1-h rest, 70-min submaximal exercise (50% VO2 peak), and 1-h recovery. Body composition was evaluated using DEXA, MRI, and MRS, and 2-h oral glucose tolerance tests with insulin monitoring were used to evaluate glucose tolerance and insulin resistance. Lipolytic and FA oxidation rates were similar during rest and recovery in all groups; however, they were lower during moderate exercise in both HIV-infected groups [glycerol Ra: HIV+RTV 5.1+/-1.2 vs. HIV+no PI 5.9+/-2.8 vs. Control 7.4+/-2.2 micromol.kg fat-free mass (FFM)-1.min-1; palmitate oxidation: HIV+RTV 1.6+/-0.8 vs. HIV+no PI 1.6+/-0.8 vs. Control 2.5+/-1.7 micromol.kg FFM.min, P<0.01]. Fasting and orally-challenged glucose and insulin values were similar among groups. Lipolytic and FA oxidation rates were blunted during moderate exercise in HIV-positive subjects taking HAART. Lower FA oxidation during exercise was primarily due to impaired plasma FA oxidation, with a minor contribution from lower nonplasma FA oxidation. Regional differences in adipose tissue lipolysis during rest and moderate exercise may be important in HIV and warrant further study

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 蛋白酶抑制剂(PI)利托那韦(RTV)与静息脂解率升高、高脂血症和胰岛素抵抗/糖耐量异常有关。本研究的目的是在接受RTV(n=12)、HAART但没有PI(n=10)和HIV血清阴性对照(n=10)的HIV阳性受试者中，研究静息、适度运动和恢复期间脂肪分解与脂肪酸(FA)氧化之间的关系，以及胰岛素敏感性/糖耐量和脂肪再分布的测量。稳定同位素示踪剂[1-(13)C]棕榈酸酯和[1，1，2，3，3-(2)H5]甘油在休息1h、次极量运动70min(50%VO2峰值)和恢复1h时持续输注血液和呼吸。使用DEXA、MRI和MRS评估身体成分，并通过2小时口服葡萄糖耐量试验和胰岛素监测来评估葡萄糖耐量和胰岛素抵抗。在静息和恢复期，所有组的脂解和FA氧化率相似，但在适度运动中，两组HIV感染者较低[甘油Ra：HIV+RTV 5.1+/-1.2 vs HIV+NO PI 5.9+/-2.8 vs对照7.4+/-2.2微摩尔·kg脱脂质量(FFM)-1.min-1；棕榈酸酯氧化：HIV+RTV 1.6+/-0.8 vs HIV+NO PI 1.6+/-0.8 vs对照2.5+/-1.7微摩尔·kg FFM.min，P&lt；0.01]。空腹和口服的血糖和胰岛素值在不同组之间相似。在服用HAART的HIV阳性受试者中，适度运动时的脂解和FA氧化率被钝化。运动中较低的FA氧化主要是由于血浆FA氧化受损，较低的非血浆FA氧化对运动有较小的贡献。休息和适度运动时脂肪组织脂解的区域差异可能是HIV的重要因素，值得进一步研究