BLUNTED LIPOLYSIS AND FATTY ACID OXIDATION DURING MODERATE EXERCISE IN HIV

HIV 患者适度运动期间脂肪分解和脂肪酸氧化减弱

• 批准号: 7953980
• 负责人: TODD CADE
• 金额: $ 0.64万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953980
• 关键词: Adipose tissue; Blood; Body Composition; Collection; Computer Retrieval of Information on Scientific Projects Database; DEXA; Exercise; Fasting; Fatty acid glycerol esters; Funding; Glucose; Glucose Intolerance; Glycerol; Grant; HIV; HIV Seropositivity; Highly Active Antiretroviral Therapy; Hyperlipidemia; Institution; Insulin; Insulin Resistance; Lipolysis; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measures; Minor; Moderate Exercise; Monitor; OGTT; Palmitates; Plasma; Protease Inhibitor; Recovery; Research; Research Personnel; Resources; Rest; Ritonavir; Source; Tracer; United States National Institutes of Health; biomedical resource; fatty acid oxidation; glucose tolerance; insulin sensitivity; oxidation; regional difference; stable isotope

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The protease inhibitor (PI) ritonavir (RTV) has been associated with elevated resting lipolytic rate, hyperlipidemia, and insulin resistance/glucose intolerance. The purpose of this study was to examine relationships between lipolysis and fatty acid (FA) oxidation during rest, moderate exercise and recovery, and measures of insulin sensitivity/glucose tolerance and fat redistribution in HIV-positive subjects taking RTV (n=12), HAART but no PI (n=10), and HIV-seronegative controls (n=10). Stable isotope tracers [1-(13)C]palmitate and [1,1,2,3,3-(2)H5]glycerol were continuously infused with blood and breath collection during 1-h rest, 70-min submaximal exercise (50% VO2 peak), and 1-h recovery. Body composition was evaluated using DEXA, MRI, and MRS, and 2-h oral glucose tolerance tests with insulin monitoring were used to evaluate glucose tolerance and insulin resistance. Lipolytic and FA oxidation rates were similar during rest and recovery in all groups; however, they were lower during moderate exercise in both HIV-infected groups [glycerol Ra: HIV+RTV 5.1+/-1.2 vs. HIV+no PI 5.9+/-2.8 vs. Control 7.4+/-2.2 micromol.kg fat-free mass (FFM)-1.min-1; palmitate oxidation: HIV+RTV 1.6+/-0.8 vs. HIV+no PI 1.6+/-0.8 vs. Control 2.5+/-1.7 micromol.kg FFM.min, P<0.01]. Fasting and orally-challenged glucose and insulin values were similar among groups. Lipolytic and FA oxidation rates were blunted during moderate exercise in HIV-positive subjects taking HAART. Lower FA oxidation during exercise was primarily due to impaired plasma FA oxidation, with a minor contribution from lower nonplasma FA oxidation. Regional differences in adipose tissue lipolysis during rest and moderate exercise may be important in HIV and warrant further study

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 蛋白酶抑制剂 (PI) 利托那韦 (RTV) 与静息脂肪分解率升高、高脂血症和胰岛素抵抗/葡萄糖耐受不良有关。本研究的目的是检查休息、适度运动和恢复期间脂肪分解和脂肪酸 (FA) 氧化之间的关系，以及接受 RTV (n=12)、HAART 但不接受 PI (n=10) 和 HIV 血清阴性对照 (n=10) 的 HIV 阳性受试者 (n=12) 的胰岛素敏感性/葡萄糖耐量和脂肪再分配的测量。在 1 小时休息、70 分钟次最大运动（50% VO2 峰值）和 1 小时恢复期间，通过血液和呼吸收集持续注入稳定同位素示踪剂 [1-(13)C]棕榈酸酯和 [1,1,2,3,3-(2)H5]甘油。使用 DEXA、MRI 和 MRS 评估身体成分，并使用 2 小时口服葡萄糖耐量试验和胰岛素监测来评估葡萄糖耐量和胰岛素抵抗。所有组在休息和恢复期间的脂肪分解和 FA 氧化率相似；然而，在两个 HIV 感染组进行适度运动时，它们都较低[甘油 Ra：HIV+RTV 5.1+/-1.2 vs. HIV+no PI 5.9+/-2.8 vs. 对照 7.4+/-2.2 micromol.kg 无脂肪质量 (FFM)-1.min-1；棕榈酸酯氧化：HIV+RTV 1.6+/-0.8 对比 HIV+无 PI 1.6+/-0.8 对比对照 2.5+/-1.7 micromol.kg FFM.min，P<0.01]。各组之间的空腹和口服葡萄糖和胰岛素值相似。接受 HAART 的 HIV 阳性受试者在适度运动期间，脂肪分解和 FA 氧化率会减弱。运动期间 FA 氧化降低主要是由于血浆 FA 氧化受损，其次是非血浆 FA 氧化降低造成的。休息和适度运动期间脂肪组织脂解的区域差异可能在艾滋病毒中很重要，值得进一步研究