INTERACTIONS OF DNA POLYMERASE WITH DNA SUBSTRATES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在皮肤癌中观察到的最常见的突变是C到T和CC到TT转换突变。一种脱氨基假说被提出来解决这些突变的产生。在这个假说中，胞嘧啶或5-甲基胞嘧啶的光产物脱氨基，形成尿嘧啶或胸腺嘧啶的光产物。光产物中的尿嘧啶或胸腺嘧啶部分优先与腺嘌呤形成Watson-Crick碱基对。在DNA复制的两个步骤后，C到T的转变突变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. C to T and CC to TT transition mutations are the most frequent mutations observed in skin cancer. A deamination hypothesis was proposed to address the generation of those mutations. In this hypothesis, a cytosine or 5-methylcytosine photoproduct deaminates to form a uracil or thymine photoproduct. Those uracil or thymine moieties in the photoproduct form Watson-Crick base-pair preferentially with adenine. After two steps of DNA replication, C to T transition mutation results.