INTERACTIONS OF DNA POLYMERASE WITH DNA SUBSTRATES

DNA 聚合酶与 DNA 底物的相互作用

• 批准号: 8361328
• 负责人: JOHN-STEPHEN Adolfino TAYLOR
• 金额: $ 2.16万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361328
• 关键词: Address; Adenine; Base Pairing; Cytosine; DNA; DNA biosynthesis; DNA-Directed DNA Polymerase; Deamination; Funding; Generations; Grant; Mass Spectrum Analysis; Methods; Mutation; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Skin Cancer; Source; Thymine; United States National Institutes of Health; Uracil; biomedical resource; cost; transition mutation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This subproject, ongoing for 15 years, addresses the use of MS methods to detect and characterize photodamage to DNA. C to T and CC to TT transition mutations are the most frequent mutations observed in skin cancer. A deamination hypothesis was proposed to address the generation of those mutations. In this hypothesis, a cytosine or 5-methylcytosine photoproduct deaminates to form a uracil or thymine photoproduct. Those uracil or thymine moieties in the photoproduct form Watson-Crick base-pair preferentially with adenine. After two steps of DNA replication, C to T transition mutation results.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这个子项目，持续了15年，解决了使用MS方法来检测和表征光损伤的DNA。 C到T和CC到TT转换突变是在皮肤癌中观察到的最常见的突变。 提出了脱氨基假说来解决这些突变的产生。 在这个假设中，胞嘧啶或5-甲基胞嘧啶光产物脱氨基形成尿嘧啶或胸腺嘧啶光产物。 光产物中的那些尿嘧啶或胸腺嘧啶部分优先与腺嘌呤形成沃森-克里克碱基对。 经过两个步骤的DNA复制，C到T转换突变的结果。