Core--Molecular imaging facility

核心——分子成像设备

• 批准号: 7804592
• 负责人: DAVID STOUT
• 金额: $ 15.78万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7804592
• 关键词: Animal Experimentation; Animal Model; Animals; Autoradiography; Award; Cancer Biology; Center Core Grants; Communities; Comprehensive Cancer Center; Consultations; Cyclotrons; Data; Data Analyses; Data Storage and Retrieval; Department of Energy; Development; Educational process of instructing; Ensure; Equipment; Future; Goals; Grant; Hour; Human Resources; Image; Imaging Device; Imaging Techniques; Imaging technology; Investigation; Link; Mus; Online Systems; Operative Surgical Procedures; Optics; Positron-Emission Tomography; Principal Investigator; Procedures; Process; Protocols documentation; Radiochemistry; Radioisotopes; Rattus; Reproduction spores; Research; Research Personnel; Resources; Rest; Retrieval; Schedule; Scheduling and Staffing; Side; Sterol O-Acyltransferase; System; Techniques; The Sun; Training; Update; Work; base; career development; design; digital; experience; imaging modality; in vivo; in vivo Cellular and Molecular Imaging Centers; instrument; instrumentation; microsystems; molecular imaging; optical imaging; programs; research study; technology/technique; tool; user-friendly

D.2.1. Introduction: The purpose of the Molecular Imaging Specialized Resource is to support the microPET, microCAT, optical and digital whole body autoradiography (DWBA) imaging requirements for the proposed ICMIC research and for additional molecular imaging projects across the rest of the UCLA biomedical community. The Molecular Imaging Specialized Resource receives support from the Department of Energy, the UCLA Jonsson Comprehensive Cancer Center Core Grant, several SPORE grants and several specialized research awards, in addition to the ICMIC. The Imaging Resource was started in 1990 as a DoE facility, with a dedicated animal research tomograph (Siemens, ACAT). We have constantly updated the imaging equipment with the newest and latest imaging technology and techniques. In 1997, the first dedicated small animal microPET system was put into routine use, followed by commercial versions from Concorde Microsystems, the P4 microPET in 1999 and the Focus microPET in 2003. As demand grew, we added a whole body digital autoradiography system (WBDA), a microCAT instrument and three optical imaging systems; two of which have both bioluminescent and fluorescent optical imaging capabilities. Since inception, over 13,000 microPET imaging experiments have been successfully conducted. In 2003, the combined number of optical, microPET, microCAT and WBDA procedures exceeded 8000 experiments. The Molecular Imaging Specialized Resource is essential for the work proposed in all four Research Components (Projects C.1-C.4), for the developmental projects of Drs. Huang and Sun (D.1 & D.2) that will extend into the next grant cycle, for future developmental projects and for career development research components. All of the projects will utilize the optical and/or microPET imaging systems for mouse and rat related research, making this resource essential and central for the entire ICMIC and for the research contained within this application. Image and data analysis will be handled by the Quantitative Analysis Specialized Resource (specialized resource 3), described in section D.3. However, all our Specialized Facilities are closely intertwined (radioisotopes prepared in the Cyclotron and Radiochemistry Specialized Resource are used for experimental studies in the Molecular Imaging Specialized Resource, and the data storage, interpretation and retrieval are facilitated by the Quantitative Analysis Specialized Resource.) The Molecular Imaging and the Quantitative Analysis facilities are highly inter-linked in their day-to-day activities to ensure that data are collected, processed and interpreted appropriately. The following table summarizes the projected needs of each ICMIC project for optical, PET, CT, optical and DWBA imaging, based on the applications found in this application and on information submitted by the principal investigators. As new developmental projects and career development researchers enter the ICMIC program, their needs will be incorporated into the operations of the facility. The goals of the Molecular Imaging Specialized Resource, in conjunction with the Quantitative Analysis Specialized Resource, are (i) to develop a fully integrated process for creating animal imaging protocols, (ii) to assist investigators in obtaining the necessary approvals for research and (iii) to produce high quality, quantitative and reproducible molecular images using both single imaging systems and combinations of imaging modalities. These goals also include developing and maintaining techniques and equipment to ensure standardized imaging and optimal data analysis. We have found that providing investigators with transparent, common tools and techniques, along with teaching the use and purposes behind these tools to new investigators, produces the most efficient use of the facility. We place special emphasis on training new investigators to become knowledgeable about molecular imaging tools and principles, so they can become capable researchers who can intelligently use the imaging techniques for both routine and new investigations. This aim is greatly aided by the synergy that comes from bringing together different investigators from different fields and backgrounds to work side-by-side in the shared imaging facility to conduct their research. Every effort in design of the work space, staffing, scheduled educational training periods, consultation practices and web-based scheduling and information accessibility is made to be user friendly; a place where both the experienced and new investigator can come and have easy access to the tools and techniques needed to complete their project. The facility is also staffed 12+ hours per day with helpful and knowledgeable personnel. At present, our support staff has a minimum of 13 years experience working with animal models and PET imaging and three years of experience with optical imaging and microCT studies. We are proud of the fact that no experiment has gone undone during the course of the current ICMIC because instrumentation, expertise or consultations were unavailable to an investigator.

D.2.1。简介：分子成像专业资源的目的是支持 拟议的 ICMIC 研究以及加州大学洛杉矶分校生物医学界其他分子成像项目的 microPET、microCAT、光学和数字全身放射自显影 (DWBA) 成像要求。除了 ICMIC 之外，分子成像专业资源还获得能源部、加州大学洛杉矶分校琼森综合癌症中心核心拨款、多项 SPORE 拨款和多项专业研究奖项的支持。成像资源中心始建于 1990 年，当时是美国能源部的一个设施，配有专用的动物研究断层扫描仪（西门子、ACAT）。我们不断用最新、最新的成像技术和工艺更新成像设备。 1997年，第一台小动物专用microPET系统投入日常使用，随后Concorde Microsystems的商业版本、1999年的P4 microPET和2003年的Focus microPET。随着需求的增长，我们增加了全身数字放射自显影系统（WBDA）、microCAT仪器和三个光学成像系统；其中两个同时具有 生物发光和荧光光学成像能力。自成立以来，已成功进行了超过 13,000 次 microPET 成像实验。 2003 年，光学、microPET、microCAT 和 WBDA 程序的实验总数超过 8000 次。 分子成像专业资源对于所有四个研究部分（项目 C.1-C.4）中提出的工作以及博士的发展项目至关重要。 Huang 和 Sun（D.1 和 D.2）将延续到下一个资助周期，用于未来的发展项目和职业发展研究组成部分。所有项目都将利用光学和/或 microPET 成像系统进行小鼠和大鼠相关研究，使得该资源对于整个 ICMIC 以及该应用中包含的研究至关重要和核心。图像和数据分析将由定量分析专业资源（专业资源 3）处理，如 D.3 节中所述。然而，我们所有的专业设施都是紧密相连的（回旋加速器和放射化学专业资源中制备的放射性同位素用于分子成像专业资源中的实验研究，定量分析专业资源促进了数据存储、解释和检索。）分子成像和定量分析设施在日常活动中高度相互关联，以确保数据的收集、处理和恢复。 适当地解释。下表根据本申请中的应用以及主要研究人员提交的信息，总结了每个 ICMIC 项目对光学、PET、CT、光学和 DWBA 成像的预计需求。作为新的开发项目 和职业发展研究人员进入 ICMIC 计划，他们的需求将被纳入该设施的运营中。分子成像专业资源与定量分析专业资源的目标是 (i) 开发用于创建动物成像方案的完全集成流程，(ii) 协助研究人员获得必要的研究批准，以及 (iii) 使用单一成像系统和成像模式组合生成高质量、定量和可重复的分子图像。这些目标还包括开发和维护技术和设备，以确保标准化成像和最佳数据分析。我们发现，为研究人员提供透明、通用的工具和技术，并教授其使用方法 对于新调查人员来说，这些工具背后的目的和目的可以最有效地利用设施。我们特别强调培训新研究人员了解分子成像工具和原理，使他们成为有能力的研究人员，能够智能地使用成像技术进行常规和新的研究。来自不同领域和背景的不同研究人员聚集在一起，在共享成像设施中并肩工作以进行研究，所产生的协同作用极大地促进了这一目标。工作空间、人员配置、预定的教育培训时间、咨询实践以及基于网络的日程安排和信息可访问性的设计尽一切努力以方便用户使用；经验丰富的和新的调查员都可以来到这里，并轻松获得完成项目所需的工具和技术。该设施还配备了乐于助人、知识渊博的人员，每天工作 12 小时以上。目前，我们的支持人员至少拥有 13 年动物模型和 PET 成像工作经验，以及 3 年光学成像和 microCT 研究经验。我们 令我们感到自豪的是，在当前的 ICMIC 过程中，没有任何实验被撤销 因为调查人员无法获得仪器、专业知识或咨询。