Core--Molecular imaging facility

核心——分子成像设备

• 批准号: 7619140
• 负责人: DAVID STOUT
• 金额: $ 15.83万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7619140
• 关键词: Animal Experimentation; Animal Model; Animals; Autoradiography; Award; Center Core Grants; Communities; Comprehensive Cancer Center; Consultations; Cyclotrons; Data; Data Analyses; Data Storage and Retrieval; Department of Energy; Development; Educational process of instructing; Ensure; Equipment; Future; Goals; Grant; Hour; Human Resources; Image; Imaging Device; Imaging Techniques; Imaging technology; Investigation; Link; Modality; Mus; Numbers; Online Systems; Operative Surgical Procedures; Optics; Positron-Emission Tomography; Principal Investigator; Procedures; Process; Protocols documentation; Purpose; Radiochemistry; Radioisotopes; Rattus; Reproduction spores; Research; Research Personnel; Resources; Rest; Retrieval; Schedule; Scheduling and Staffing; Side; Sterol O-Acyltransferase; System; Techniques; Training; Update; Work; base; career; day; design; digital; experience; in vivo Cellular and Molecular Imaging Centers; instrument; instrumentation; microsystems; molecular imaging; optical imaging; programs; research and development; research study; technology/technique; tool; user-friendly

D.2.1. Introduction: The purpose of the Molecular Imaging Specialized Resource is to support the microPET, microCAT, optical and digital whole body autoradiography (DWBA) imaging requirements for the proposed ICMIC research and for additional molecular imaging projects across the rest of the UCLA biomedical community. The Molecular Imaging Specialized Resource receives support from the Department of Energy, the UCLA Jonsson Comprehensive Cancer Center Core Grant, several SPORE grants and several specialized research awards, in addition to the ICMIC. The Imaging Resource was started in 1990 as a DoE facility, with a dedicated animal research tomograph (Siemens, ACAT). We have constantly updated the imaging equipment with the newest and latest imaging technology and techniques. In 1997, the first dedicated small animal microPET system was put into routine use, followed by commercial versions from Concorde Microsystems, the P4 microPET in 1999 and the Focus microPET in 2003. As demand grew, we added a whole body digital autoradiography system (WBDA), a microCAT instrument and three optical imaging systems; two of which have both bioluminescent and fluorescent optical imaging capabilities. Since inception, over 13,000 microPET imaging experiments have been successfully conducted. In 2003, the combined number of optical, microPET, microCAT and WBDA procedures exceeded 8000 experiments. The Molecular Imaging Specialized Resource is essential for the work proposed in all four Research Components (Projects C.1-C.4), for the developmental projects of Drs. Huang and Sun (D.1 & D.2) that will extend into the next grant cycle, for future developmental projects and for career development research components. All of the projects will utilize the optical and/or microPET imaging systems for mouse and rat related research, making this resource essential and central for the entire ICMIC and for the research contained within this application. Image and data analysis will be handled by the Quantitative Analysis Specialized Resource (specialized resource 3), described in section D.3. However, all our Specialized Facilities are closely intertwined (radioisotopes prepared in the Cyclotron and Radiochemistry Specialized Resource are used for experimental studies in the Molecular Imaging Specialized Resource, and the data storage, interpretation and retrieval are facilitated by the Quantitative Analysis Specialized Resource.) The Molecular Imaging and the Quantitative Analysis facilities are highly inter-linked in their day-to-day activities to ensure that data are collected, processed and interpreted appropriately. The following table summarizes the projected needs of each ICMIC project for optical, PET, CT, optical and DWBA imaging, based on the applications found in this application and on information submitted by the principal investigators. As new developmental projects and career development researchers enter the ICMIC program, their needs will be incorporated into the operations of the facility. The goals of the Molecular Imaging Specialized Resource, in conjunction with the Quantitative Analysis Specialized Resource, are (i) to develop a fully integrated process for creating animal imaging protocols, (ii) to assist investigators in obtaining the necessary approvals for research and (iii) to produce high quality, quantitative and reproducible molecular images using both single imaging systems and combinations of imaging modalities. These goals also include developing and maintaining techniques and equipment to ensure standardized imaging and optimal data analysis. We have found that providing investigators with transparent, common tools and techniques, along with teaching the use and purposes behind these tools to new investigators, produces the most efficient use of the facility. We place special emphasis on training new investigators to become knowledgeable about molecular imaging tools and principles, so they can become capable researchers who can intelligently use the imaging techniques for both routine and new investigations. This aim is greatly aided by the synergy that comes from bringing together different investigators from different fields and backgrounds to work side-by-side in the shared imaging facility to conduct their research. Every effort in design of the work space, staffing, scheduled educational training periods, consultation practices and web-based scheduling and information accessibility is made to be user friendly; a place where both the experienced and new investigator can come and have easy access to the tools and techniques needed to complete their project. The facility is also staffed 12+ hours per day with helpful and knowledgeable personnel. At present, our support staff has a minimum of 13 years experience working with animal models and PET imaging and three years of experience with optical imaging and microCT studies. We are proud of the fact that no experiment has gone undone during the course of the current ICMIC because instrumentation, expertise or consultations were unavailable to an investigator.

D.2.1。简介：分子影像专业资源的目的是支持 MicroPET、microCAT、光学和数字全身放射自显影(DWBA)成像要求用于拟议的ICMIC研究和加州大学洛杉矶分校生物医学界其他其他分子成像项目。除了ICMIC外，分子成像专业资源还得到了能源部、加州大学洛杉矶分校琼森综合癌症中心核心赠款、几个孢子赠款和几个专门研究奖项的支持。影像资源中心成立于1990年，当时是美国能源部的一家机构，拥有一台专门的动物研究断层扫描仪(西门子，ACAT)。我们不断更新成像设备，采用最新和最新的成像技术和技术。1997年，第一个专用的小动物微型PET系统投入常规使用，随后协和微系统公司的商业版本，1999年的P4微型PET和2003年的Focus微型PET。随着需求的增长，我们增加了一个全身数字放射自显影系统(WBDA)、一个微型CAT仪器和三个光学成像系统；其中两个都有 生物发光和荧光光学成像能力。自成立以来，已成功进行了13,000多个微型PET成像实验。2003年，光学、微型PET、微型CAT和WBDA程序的总数超过了8000个实验。 分子成像专业资源对于所有四个研究组成部分(C.1-C.4项目)、将延伸到下一个赠款周期的黄博士和孙博士的发展项目(D.1和D.2)、未来发展项目和职业发展研究组成部分中提议的工作至关重要。所有项目都将利用光学和/或微型PET成像系统进行与小鼠和大鼠相关的研究，使这一资源成为整个ICMIC和这一应用所包含的研究的关键和核心。图像和数据分析将由D.3节所述的量化分析专业资源(专业资源3)处理。然而，我们所有的专业设施都是紧密交织在一起的(回旋加速器和放射化学专业资源中制备的放射性同位素用于分子成像专业资源中的实验研究，定量分析专业资源为数据存储、解释和检索提供便利)。分子成像和定量分析设施在日常活动中高度相互联系，以确保适当地收集、处理和解释数据。下表汇总了每个ICMIC项目对光学、PET、CT、光学和DWBA成像的预测需求，这些需求基于本申请中的应用和主要调查人员提交的信息。作为新的发展项目 和职业发展研究人员进入ICMIC计划，他们的需求将被纳入设施的运营。分子成像专业资源与定量分析专业资源的目标是：(I)开发一个创建动物成像方案的完全集成的过程，(Ii)协助研究人员获得研究所需的批准，以及(Iii)使用单一成像系统和成像模式的组合来产生高质量、定量和可重现的分子图像。这些目标还包括开发和维护技术和设备，以确保标准化成像和最佳数据分析。我们发现，为调查人员提供透明、通用的工具和技术，以及教授使用 这些工具背后的目的和目的使新的调查人员能够最有效地利用设施。我们特别重视培训新的研究人员，使他们了解分子成像工具和原理，这样他们就可以成为有能力的研究人员，能够智能地将成像技术用于常规和新的研究。这一目标在很大程度上得益于将来自不同领域和背景的不同研究人员聚集在一起，在共享的成像设施中并肩工作以进行他们的研究的协同效应。在工作空间、人员配置、预定的教育培训周期、咨询做法以及基于网络的日程安排和信息获取方面所做的一切努力都是对用户友好的；一个有经验的和新的调查员都可以来的地方，可以很容易地获得完成项目所需的工具和技术。该设施还配备了每天12小时以上的工作人员，以及乐于助人和知识渊博的人员。目前，我们的支持人员至少有13年的动物模型和PET成像工作经验，以及3年的光学成像和微CT研究经验。我们 我们感到自豪的是，在本届ICMIC期间，没有一项实验失败 因为调查员无法获得仪器、专业知识或咨询。