COBRE: UKS: CORE B: HIGH THROUGHPUT SCREENING & TARGET IDENTIFICATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 高通量筛选（HTS）已成为生物医学研究的有力工具和药物发现的资源。HTS的目的是筛选大量与生物靶点选择性相互作用的小分子，以便它们可以用作探针来了解新表征的蛋白质的功能，并分析生理过程，细胞现象和疾病机制。堪萨斯大学的HTS实验室（www.hts.ku.edu）是通过COBRE赠款建立的，并为COBRE研究人员以及当地和国家研究社区提供服务。我们已经并将继续支持COBRE研究人员，并将扩大我们高通量筛选的范围和能力，以满足生物医学研究人员日益增长的需求。我们的目标是（1）加强我们的检测开发能力，以进口和调整基于生化和细胞的检测，以HTS格式和（2）加强我们的高通量筛选能力，以进行有效的筛选活动。我们与个别研究人员合作，调整他们的台式试验以适应HTS操作，并使用这些成功调整的HTS试验来筛选我们现有的小有机化合物化学库中显示生物活性的分子。由于现有的HTS设施和人员在堪萨斯大学，我们相信，进一步扩大将作出重大贡献，对新的有趣的化合物的发现，并对设计，合成和评估衍生自原始命中来自HTS的类似物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. High throughput screening (HTS) has become a powerful tool for biomedical research and a resource for drug discovery. The purpose of HTS is to screen large numbers of small molecules that interact selectively with biological targets so that they may be used as probes to understand the function of newly characterized proteins and to analyze physiological e processes, cellular phenomena, and disease mechanisms. The HTS laboratory at the University of Kansas (www.hts.ku.edu) was established through the COBRE grant and has provided service to COBRE investigators, and the local and national research community. We have supported and will continue to support COBRE investigators and will also expand both the scope and capacity of our high throughput screening to meet the increased needs of biomedical researchers. Our objectives are (1) to strengthen our assay development capacity to import and adapt biochemical and cell-based assays to HTS format and (2) to strengthen our high throughput screening capacity to carry out efficient screening campaigns. We work with individual research investigators to adapt their bench-top assays to suit HTS operation and use these successfully adapted HTS assays to screen our existing chemical library of small organic compounds for molecules showing biological activity. Because of the existing HTS facility and personnel at the University of Kansas, we are certain that further expansion will make significant contributions toward the discovery of new interesting compounds and toward the design, synthesis and evaluation of analogues derived from original hits coming from HTS.