Anemia in the Elderly: Pathogenesis

老年人贫血：发病机制

• 批准号: 7797541
• 负责人: JEFFREY S FRIEDMAN
• 金额: $ 37.78万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7797541
• 关键词: Accounting; Age; Age-Years; Anemia; Cell Aging; Chronic; Elderly; Erythrocyte Membrane; Erythrocyte Survival; Erythrocytes; Erythroid; Erythroid Progenitor Cells; Hemoglobin; Hemoglobin concentration result; Individual; Infection; Interleukin-6; Kidney Failure; Length; Longevity; Malignant Neoplasms; Measurement; Measures; Mitochondrial DNA; Nature; Pathogenesis; Patients; Phosphatidylserines; Population; Production; Proteins; Serum; Structure; Testosterone; Variant; aged; glutathione peroxidase; granulocyte; hexokinase; male; men; normal aging; older patient; oxidative damage; peripheral blood; progenitor; telomere

DESCRIPTION (provided by applicant): Anemia is common in the elderly, often as a result of known causes such as renal failure, cancer, or chronic infection. But even when patients with known causes are eliminated from consideration, hemoglobin levels decline during ageing. We will study such patients over the age of 75 and compare those whose with hemoglobin levels in the highest decile with those in the lowest decile. Patients aged 30 to 40 years will serve as controls. The elderly patients and controls will be studied for evidence of erythropoietic progenitor cell (EPC) senescence and for abnormalities of circulating erythrocytes. With circulating granulocytes serving as surrogates for EPC we will examine mtDNA and telomere length and G loop structure. Erythrocyte survival will be estimated by CO levels and red cell hexokinase activity; oxidative damage to erythrocytes will be assessed by estimating GSSG/GSH, glutathione peroxidase, carbonyl proteins, TEARS; exposure of phosphatidylserine on erythrocyte membranes will be documented. Serum IL-6 measurements and in males serum testosterone levels will be determined. Parameters that show differences between the highest and lowest deciles will be candidate causes of anemia in the elderly. If such differences also occur among controls they could account for the broad normal variation in hemoglobin levels.

描述（由申请人提供）：贫血在老年人中很常见，通常是由于已知的原因，如肾衰竭、癌症或慢性感染。但是，即使排除了已知病因的患者，血红蛋白水平也会随着年龄的增长而下降。我们将研究75岁以上的此类患者，并将血红蛋白水平处于最高十分位数与处于最低十分位数的患者进行比较。30 - 40岁的患者将作为对照。将研究老年患者和对照组的红细胞生成祖细胞（EPC）衰老和循环红细胞异常的证据。循环粒细胞作为EPC的替代品，我们将检查mtDNA和端粒长度和G环结构。将通过CO水平和红细胞己糖激酶活性估计红细胞存活;将通过估计GSSG/GSH、谷胱甘肽过氧化物酶、羰基蛋白、TEARS评估红细胞的氧化损伤;将记录红细胞膜上磷脂酰丝氨酸的暴露。将测定血清IL-6测量值和雄性血清睾酮水平。显示最高和最低十分位数之间差异的参数将是老年人贫血的候选原因。如果这种差异也发生在对照组中，那么它们可以解释血红蛋白水平的广泛正常变化。