Low-Cost, Onsite Biomarker Test Kits for Alcohol-Associated Disorders (Phoenix)

磷脂酰乙醇（PEth）作为长期饮酒的标志物受到特别关注。目前分析PEth的方法是基于高效液相色谱-质谱法（HPLC-MS）。最近，芬兰的研究人员已经成功地产生了针对PEth的单克隆抗体，并展示了基于实验室的荧光PEth测定和对掺入PEth的人红细胞样品的FACS分析。这种基于实验室的方法仍然需要昂贵的仪器和熟练的人员来进行分析。用于检测的快速测定法的不可用和测试的标准化的缺乏阻碍了这些生物标志物的临床使用。为了克服昂贵的仪器在PEth分析的局限性，我们建议开发和证明的可行性，低成本，现场测定PEth使用单克隆抗体的电化学免疫传感器技术。

Phosphatidylethanol (PEth) has gained special attention as a marker of long-term alcohol drinking. Current methods of analysis of PEth are based on the high performance liquid chromatography-mass spectrometric methods (HPLC-MS). More recently researchers in Finland have successfully generated monoclonal antibodies to PEth and have demonstrated a laboratory based fluorescent PEth assay and FACS analysis of human red blood cell samples spiked with PEth. This laboratory-based method still requires expensive instrumentation and skilled personnel to perform the analysis. Unavailability of rapid assays for the detection and the lack of standardization of the tests have hampered the clinical use of these biomarkers. To overcome the limitation of expensive instrumentation in PEth analysis we propose to develop and demonstrate the feasibility of a low-cost, onsite assay for PEth using a monoclonal antibody based electrochemical immunosensor technology.