Improved methodologies to measure novel alcohol biomarkers in whole blood

改进的方法来测量全血中新型酒精生物标志物

• 批准号: 8183977
• 负责人: KUMAR SUBRAMANIAN
• 金额: $ 21.05万
• 依托单位: PHOENIX BIOSYSTEM, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8183977
• 关键词: Affect; Age; Alcohol abuse; Alcohol consumption; Alcohols; Biological; Biological Assay; Biological Markers; Blood; Body mass index; Clinical; Detection; Enzyme Tests; Enzyme-Linked Immunosorbent Assay; Ethanol; Foundations; Gamma-glutamyl transferase; Gender; Goals; Half-Life; High Pressure Liquid Chromatography; Intake; Liver; Measurable; Measures; Metabolic; Methodology; Methods; Organ; Patients; Phase; Phospholipase; Phospholipase D; Phospholipids; Physicians; Property; Recording of previous events; Research; Research Personnel; Sampling; Specificity; Standardization; Testing; Tissues; Validation; Whole Blood; Work; alcohol use disorder; base; carbohydrate-deficient transferrin; improved; mean corpuscular volume observed; novel; phosphatidylethanol; problem drinker; response; sobriety; tool

DESCRIPTION (provided by applicant): Phosphatidylethanol (PEth) is a phospholipid formed only in the presence of ethanol via the action of phospholipase D. It has been measured in whole blood and proposed as a marker of ethanol abuse due to its high specificity and low degradation rate. The mean half-life of PEth in blood from alcoholics was found to be 4 days, and PEth was still measurable after 2 weeks of sobriety. Our overall goal is to expand on our successful preliminary work and develop a sensitive, high throughput ELISA biomarker assay for PEth analysis in whole blood. Current methods of analysis of PEth are based on the high performance liquid chromatography-mass spectrometric methods. Unavailability of rapid assays for the detection and the lack of standardization of the tests have hampered the clinical use of these biomarkers. The final PEth ELISA product that would result from this application could have a significant impact in the study and management of alcohol use disorders. PUBLIC HEALTH RELEVANCE: Our overall goal is to expand on our successful preliminary work and develop a sensitive, high throughput ELISA biomarker assay for PEth analysis in whole blood.

描述（由申请人提供）：磷脂酰乙醇（PEth）是一种仅在乙醇存在下通过磷脂酶 D 的作用形成的磷脂。它已在全血中进行测量，并由于其高特异性和低降解率而被提议作为乙醇滥用的标志物。研究发现，酗酒者血液中 PEth 的平均半衰期为 4 天，并且在清醒两周后仍可测量到 PEth。我们的总体目标是扩展我们成功的前期工作，开发一种灵敏、高通量的 ELISA 生物标志物检测方法，用于全血 PEth 分析。目前的 PEth 分析方法基于高效液相色谱-质谱法。无法进行快速检测以及测试缺乏标准化阻碍了这些生物标志物的临床使用。该应用产生的最终 PEth ELISA 产品可能会对酒精使用障碍的研究和管理产生重大影响。 公共卫生相关性：我们的总体目标是扩展我们成功的前期工作，并开发一种灵敏、高通量的 ELISA 生物标志物检测方法，用于全血中的 PEth 分析。