Improved methodologies to measure novel alcohol biomarkers in whole blood

描述（由申请人提供）：磷脂酰乙醇（PEth）是一种仅在乙醇存在下通过磷脂酶d的作用形成的磷脂。由于其高特异性和低降解率，已在全血中测量并建议作为乙醇滥用的标志。酗酒者血液中PEth的平均半衰期为4天，并且在清醒2周后仍可测量到PEth。我们的总体目标是扩展我们成功的初步工作，并开发一种灵敏，高通量的ELISA生物标志物检测全血中PEth的分析。目前的分析方法是基于高效液相色谱-质谱联用方法。缺乏快速检测方法和缺乏标准化的检测方法阻碍了这些生物标志物的临床应用。最终的PEth酶联免疫吸附试验产品将在酒精使用障碍的研究和管理中产生重大影响。

DESCRIPTION (provided by applicant): Phosphatidylethanol (PEth) is a phospholipid formed only in the presence of ethanol via the action of phospholipase D. It has been measured in whole blood and proposed as a marker of ethanol abuse due to its high specificity and low degradation rate. The mean half-life of PEth in blood from alcoholics was found to be 4 days, and PEth was still measurable after 2 weeks of sobriety. Our overall goal is to expand on our successful preliminary work and develop a sensitive, high throughput ELISA biomarker assay for PEth analysis in whole blood. Current methods of analysis of PEth are based on the high performance liquid chromatography-mass spectrometric methods. Unavailability of rapid assays for the detection and the lack of standardization of the tests have hampered the clinical use of these biomarkers. The final PEth ELISA product that would result from this application could have a significant impact in the study and management of alcohol use disorders. PUBLIC HEALTH RELEVANCE: Our overall goal is to expand on our successful preliminary work and develop a sensitive, high throughput ELISA biomarker assay for PEth analysis in whole blood.