BACTERIAL CELL-CELL SIGNALING AND COMMUNITY SUCCESSION IN BIOFILMS

生物膜中的细菌细胞间信号传导和群落演替

• 批准号: 7953856
• 负责人: Tiffany Van Mooy
• 金额: $ 0.56万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953856
• 关键词: Algae; Bacteria; Cell Communication; Cells; Cellular Structures; Chemicals; Chromatography; Communities; Computer Retrieval of Information on Scientific Projects Database; Development; Eukaryota; Funding; Future; Grant; Growth; Individual; Institution; Laboratories; Marines; Mass Spectrum Analysis; Measures; Methods; Microbial Biofilms; Nucleic Acids; Organism; Performance; Physiological; Research; Research Personnel; Resolution; Resources; Shipping; Ships; Signal Transduction; Signaling Molecule; Source; Staging; Surface; Taxon; Technology; Testing; Thoracica; United States; United States National Institutes of Health; analytical tool; base; cell growth; cost; efficacy testing; intercellular communication; novel; prevent; user-friendly

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biofouling of ship hulls costs the United States Navy hundreds of millions of dollars each year in lost fuel efficiency. Although eukaryotic biofoulers, such as algae and barnacles, are responsible for the majority of drag that limits hull performance, the stage for colonization by these organisms is set by the development of bacterial biofilms on the hull surface. Recent studies have shown that several abundant groups of marine bacteria secrete chemical signals that are used to induce extraordinary coordination between cells on submerged surfaces; when conditions are optimal these signaling molecules trigger the growth of cell structures that transform individual attached bacterial cells to a true three-dimensional bacterial biofilm. The discovery of these cell-cell communications has led to the hypotheses that developing biofilms are populated by a tightly-orchestrated succession of different bacterial groups and that eukaryotic biofoulers may "eavesdrop" on signaling between bacterial cells to optimize their chances for recruitment on the biofilm. If these hypotheses are true, arresting the development of just one bacterial group in the succession has the potential to slow, or even possibly prevent, the recruitment of drag-causing eukaryotes. Although the current understanding of cell-cell signaling in bacterial biofilms has been gained primarily through cultivation of pure species of bacteria in the laboratory, the taxonomic and physiological diversity of marine bacterial communities warrants an additional approach that is free of the limits and biases of cell cultivation. Here, I propose to apply novel, cultivation-independent, chromatography/mass spectrometry-based technologies to measure taxon-specific cell growth and to test the hypotheses that: 1) there is a regular succession of bacterial groups in marine biofilms on ship's hulls, and 2) this succession is regulated by cell-cell signaling molecules. These technologies, are reliable, quantitative, and have taxonomic resolution similar to nucleic acids-based methods that are often more cumbersome and less quantitative. Once fully developed, these approaches will provide a powerful and user-friendly analytical tool to test the efficacy of current and future anti-fouling technologies on retarding the growth of specific bacterial taxa contained in biofilms.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 船体生物污染每年给美国海军造成数亿美元的燃油效率损失。尽管藻类和藤壶等真核生物污垢是限制船体性能的大部分阻力的原因，但这些生物体定植的阶段是由细菌的发育决定的。 船体表面的生物膜。最近的研究表明，几种丰富的海洋细菌群会分泌化学信号，用于诱导水下表面细胞之间的非凡协调。当条件最佳时，这些信号分子会触发细胞结构的生长，将单个附着的细菌细胞转化为真正的三维细菌生物膜。这些细胞间通讯的发现引发了这样的假设：正在发育的生物膜由一系列紧密协调的不同细菌群组成，真核生物污垢可能“窃听”细菌细胞之间的信号传导，以优化它们在生物膜上招募的机会。如果这些假设成立，那么连续阻止一个细菌群的发育就有可能减缓甚至可能阻止引起耐药的真核生物的招募。 尽管目前对细菌生物膜中细胞间信号传导的了解主要是通过在实验室培养纯种细菌获得的，但海洋细菌群落的分类学和生理多样性需要一种不受细胞培养限制和偏见的额外方法。在这里，我建议应用新颖的、独立于培养的、基于色谱/质谱的技术来测量分类单元特异性细胞生长，并测试以下假设：1）船体上的海洋生物膜中存在规则的细菌群演替，2）这种演替受到细胞间信号分子的调节。这些技术可靠、定量，并且具有类似于基于核酸的方法的分类分辨率，而基于核酸的方法通常更麻烦且定量性较差。一旦完全开发出来，这些方法将提供强大且用户友好的分析工具，以测试当前和未来的防污技术在延缓生物膜中特定细菌类群生长方面的功效。