ENDOCRINE DISRUPTION BY ORGANOTINS IN OBESITY AND DIABETES

有机锡对肥胖和糖尿病的内分泌干扰

• 批准号: 7956521
• 负责人: BRUCE BLUMBERG
• 金额: $ 0.27万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956521
• 关键词: Bromodeoxyuridine; Cardiovascular Diseases; Cell Culture Techniques; Cell Line; Cells; Chloride Ion; Chlorides; Computer Retrieval of Information on Scientific Projects Database; Diabetes Mellitus; Disease; Endocrine disruption; Environmental Monitoring; Fluorescence; Funding; Grant; Hypertension; Immunohistochemistry; Institution; Laboratories; Lipids; Metabolic syndrome; Modeling; Mus; Obesity; Protocols documentation; Research; Research Personnel; Resources; Source; Staining method; Stains; Testing; United States National Institutes of Health; adipocyte differentiation; in vivo; tributyltin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Test an environmental obesogen model for relevance to obesity and metabolic syndrome diseases (diabetes, hypertension, cardiovascular disease). Specifically, we will test whether organotins (e.g. tributyltin chloride) are able to promote adipocyte differentiation and lipid accumulation in preadipocyte cell lines, primary mouse cell cultures and in vivo using fluorescence immunohistochemistry. The LFD will assist in establishing protocols for co-localization of cell markers (Hoechst, anti-BrdU, lipid stains).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 测试环境致肥胖模型与肥胖和代谢综合征疾病（糖尿病、高血压、心血管疾病）的相关性。具体来说，我们将使用荧光免疫组织化学测试有机锡（例如氯化三丁基锡）是否能够促进前脂肪细胞系、原代小鼠细胞培养物和体内的脂肪细胞分化和脂质积累。 LFD 将协助建立细胞标记物（Hoechst、抗 BrdU、脂质染色剂）共定位的方案。