ENDOCRINE DISRUPTION BY ORGANOTINS IN OBESITY AND DIABETES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 测试环境肥胖模型与肥胖和代谢综合征疾病(糖尿病、高血压、心血管疾病)的相关性。具体地说，我们将使用荧光免疫组织化学方法测试有机锡(例如三丁基锡)是否能够促进前脂肪细胞系、原代小鼠细胞培养和体内脂肪细胞的分化和脂肪积累。LFD将协助建立细胞标志物(Hoechst、抗BrdU、脂类染色)的共同定位方案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Test an environmental obesogen model for relevance to obesity and metabolic syndrome diseases (diabetes, hypertension, cardiovascular disease). Specifically, we will test whether organotins (e.g. tributyltin chloride) are able to promote adipocyte differentiation and lipid accumulation in preadipocyte cell lines, primary mouse cell cultures and in vivo using fluorescence immunohistochemistry. The LFD will assist in establishing protocols for co-localization of cell markers (Hoechst, anti-BrdU, lipid stains).