ENDOCRINE DISRUPTION BY ORGANOTINS IN OBESITY AND DIABETES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 测试环境肥胖模型与肥胖和代谢综合征疾病（糖尿病、高血压、心血管疾病）的相关性。具体而言，我们将测试是否有机锡（如氯化三丁基锡）能够促进脂肪细胞分化和脂质积累的前脂肪细胞系，原代小鼠细胞培养和体内使用荧光免疫组织化学。LFD将协助建立细胞标志物（Hoechst、抗BrdU、脂质染色）共定位方案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Test an environmental obesogen model for relevance to obesity and metabolic syndrome diseases (diabetes, hypertension, cardiovascular disease). Specifically, we will test whether organotins (e.g. tributyltin chloride) are able to promote adipocyte differentiation and lipid accumulation in preadipocyte cell lines, primary mouse cell cultures and in vivo using fluorescence immunohistochemistry. The LFD will assist in establishing protocols for co-localization of cell markers (Hoechst, anti-BrdU, lipid stains).