DETECTION OF VULNERABLE PLAQUES USING RAMAN, FLUORESCENCE AND REFLECTANCE SPECT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 易损斑块的形态特征为：纤细的纤维帽、大的坏死核、浅层泡沫细胞和斑块内出血。我们建议结合使用多种光谱方法，包括拉曼光谱、漫反射光谱和本征荧光光谱，来检测这些易损斑块的关键形态标志。我们将这种组合技术称为多模式光谱学(MMS)。本文对17例人颈动脉标本进行了体外初步研究。在获得光谱后，对每个标本进行组织学评估。确定了从漫反射光谱获得的参数，即血红蛋白浓度，可以潜在地用于检测斑块内出血。从本征荧光中提取的与组织最顶层中的胶原蛋白含量有关的参数可以用来检测薄纤维帽的存在。最后，与胆固醇和坏死物的量有关的拉曼光谱参数可能被用来检测坏死核。MMS方法很有希望提供斑块成分的补充形态信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Vulnerable plaques are characterized by morphological features such as a thin fibrous cap, a large necrotic core, superficial foam cells, and intraplaque hemorrhage. We propose the use of a combination of multiple spectroscopic methods, including Raman, diffuse reflectance, and intrinsic fluorescence spectroscopy, to detect these key morphological markers of vulnerable plaque. We refer to the combined technique as multimodal spectroscopy (MMS). A preliminary study was performed in vitro on 17 human carotid artery specimens. Following the acquisition of the spectra, each specimen was histologically evaluated. It was determined that parameters obtained from diffuse reflectance spectroscopy, the hemoglobin concentration, can potentially be used to detect intraplaque hemorrhage. A parameter extracted from the intrinsic fluorescence that relates to the amount of collagen in the topmost layers of the tissue can be used to sense the presence of a thin fibrous cap. Finally, a Raman spectroscopy parameter related to the amount of cholesterol and necrotic material can potentially be used to detect a necrotic core. The MMS method shows great promise to provide complementary morphological information about plaque composition.