DEVELOPMENT OF RAMAN CLINICAL INSTRUMENT FOR TRANSCUTANEOUS GLUCOSE DETECTION

经皮血糖检测拉曼临床仪器的研制

• 批准号: 8170409
• 负责人: CHULHO KONG
• 金额: $ 1.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170409
• 关键词: Animal Model; Area; Biological; Blood Glucose; Clinical; Collection; Computer Retrieval of Information on Scientific Projects Database; Detection; Development; Devices; Excision; Fiber; Fiber Optics; Fluorescence; Funding; Glucose; Goals; Gold; Grant; Institution; Lasers; Light; Measurement; Raman Spectrum Analysis; Research; Research Personnel; Resources; Signal Transduction; Source; System; Tissues; United States National Institutes of Health; base; data acquisition; design; flexibility; in vivo; instrument; tissue phantom; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the proposed subproject is to establish Raman spectroscopy as a viable clinical tool for in vivo measurement of blood glucose, based on our previous successful application in tissue phantoms and animal models. However, the spectral signals emanating from biological tissue are often weak, and therefore efficient collection is essential. Only approximately 1010 of the incident light is Raman scattered, severely limiting data-acquisition rates. The excitation light power that can be delivered to a given area of tissue is limited by undesirable effects such as overheating. It is therefore important to maximize the light collected. While we were able to significantly enhance light collection efficiency in a bench-top system using an off-axis gold coated paraboloidal mirror, the footprint requirements of the clinical instrument necessitate the use of a fiber optic-based excitation and collection device. To accomplish this, we have designed a compact spectroscopic system, that incorporates a tunable laser and a broadband source (which are critical for fluorescence removal and turbidity correction, respectively), alongside a flexible fiber probe capped with a compound parabolic concentrator (CPC).

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该子项目的目标是建立拉曼光谱作为一个可行的临床工具，在体内测量血糖，基于我们以前的成功应用在组织模型和动物模型。然而，从生物组织发出的光谱信号通常很弱，因此有效的收集是必不可少的。只有大约1010的入射光是拉曼散射，严重限制了数据采集速率。可以传递到组织的给定区域的激发光功率受到诸如过热的不期望的效应的限制。因此，重要的是最大限度地收集光。虽然我们能够使用离轴镀金抛物面镜显著提高台式系统的光收集效率，但临床仪器的占地面积要求需要使用基于光纤的激发和收集装置。为了实现这一点，我们设计了一个紧凑的光谱系统，它结合了可调谐激光器和宽带源（这是至关重要的荧光去除和浊度校正，分别），以及一个灵活的光纤探头与复合抛物面聚光器（CPC）。