DEVELOPMENT OF RAMAN CLINICAL INSTRUMENT FOR TRANSCUTANEOUS GLUCOSE DETECTION

经皮血糖检测拉曼临床仪器的研制

• 批准号: 8364151
• 负责人: CHULHO KONG
• 金额: $ 2.97万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364151
• 关键词: Animal Model; Area; Biological; Biomedical Research; Blood Glucose; Clinical; Collection; Detection; Development; Devices; Excision; Fiber; Fiber Optics; Fluorescence; Funding; Glucose; Goals; Gold; Grant; Lasers; Light; Measurement; National Center for Research Resources; Principal Investigator; Raman Spectrum Analysis; Research; Research Infrastructure; Resources; Signal Transduction; Source; System; Tissues; United States National Institutes of Health; base; cost; data acquisition; design; flexibility; in vivo; instrument; tissue phantom; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the proposed subproject is to establish Raman spectroscopy as a viable clinical tool for in vivo measurement of blood glucose, based on our previous successful application in tissue phantoms and animal models. However, the spectral signals emanating from biological tissue are often weak, and therefore efficient collection is essential. Only approximately 1010 of the incident light is Raman scattered, severely limiting data-acquisition rates. The excitation light power that can be delivered to a given area of tissue is limited by undesirable effects such as overheating. It is therefore important to maximize the light collected. While we were able to significantly enhance light collection efficiency in a bench-top system using an off-axis gold coated paraboloidal mirror, the footprint requirements of the clinical instrument necessitate the use of a fiber optic-based excitation and collection device. To accomplish this, we have designed a compact spectroscopic system, that incorporates a tunable laser and a broadband source (which are critical for fluorescence removal and turbidity correction, respectively), alongside a flexible fiber probe capped with a compound parabolic concentrator (CPC).

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 拟议子项目的目标是基于我们之前在组织模型和动物模型中的成功应用，将拉曼光谱建立为体内血糖测量的可行临床工具。然而，从生物组织发出的光谱信号通常很弱，因此有效的收集至关重要。只有大约 1010 的入射光被拉曼散射，严重限制了数据采集率。可以传递到给定组织区域的激发光功率受到诸如过热之类的不良影响的限制。因此，最大化收集的光非常重要。虽然我们能够使用离轴镀金抛物面镜显着提高台式系统中的光收集效率，但临床仪器的占地面积要求需要使用基于光纤的激发和收集设备。为了实现这一目标，我们设计了一个紧凑的光谱系统，其中包含可调谐激光器和宽带光源（分别对于荧光去除和浊度校正至关重要），以及带有复合抛物面聚光器（CPC）的柔性光纤探头。