VIRUS SWELLING AND MATURATION DYNAMICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在过去的五年中，通过X射线结晶学和低温电子显微镜(EM)实验确定的病毒结构的数量急剧增加，包括病毒衣壳采用了几种功能相关的构象。此外，通过时间分辨的小角散射实验，现在可以监测病毒的动力学，对于一些系统，已经观察到了几个中间体。这些大规模的重排是病毒生命周期中不可或缺的一部分，在成熟过程中观察到，在组装过程中，可能参与了感染期间RNA的释放。破坏病毒的组装或成熟过程可能是抗病毒活性的潜在靶点。因此，描述、预测和探索病毒的这种构象变化对于了解其活性机制是必不可少的，并将有助于新药的开发。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the past five years there has been a tremendous growth of the number of virus structures solved by X-ray crystallography and cryo electron microscopy (EM) experiments, including virus capsids adopting several functionally relevant conformations. In addition, with time resolved small angle scattering experiments dynamics of viruses can now be monitored and for some systems several intermediate have been observed. These large-scale rearrangements are an integral part of the life cycle of the virus and have been observed in the maturation process, during assembly and may be involved in the release of RNA during infection. Disrupting the assembly or the maturation processes in viruses may be potential targets for antiviral activities. Thus, the description, prediction and exploration of such conformational changes for viruses are essential to understand their mechanism of activity and should be helpful in the development of new drugs.