SIMULTANEOUS QUANTIFICATION OF GLUTAMATE AND GLUTAMINE USING CT-PRESS AT 3T

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 谷氨酸和谷氨酰胺是人脑中的两种主要代谢产物。谷氨酸和谷氨酰胺的准确定量对于理解脑代谢至关重要，并有可能揭示各种神经元疾病的机制。不幸的是，使用NMR定量谷氨酸和谷氨酰胺两者是困难的，因为它们的光谱完全由耦合共振组成并且彼此重叠。最近，具有优化时序的CT-PRESS已被用于检测具有塌陷单峰的谷氨酸。然而，由于与谷氨酸和NAA两者重叠，使用该技术不能解析谷氨酰胺共振。在这项工作中，提出了一种方法，实现定量的谷氨酸和谷氨酰胺，并应用于体内CT-PRESS光谱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glutamate and glutamine are two major metabolites in human brain. Accurate quantification of both glutamate and glutamine is essential for understanding brain metabolism and has the potential to reveal mechanisms of various neuronal diseases. Unfortunately, quantification of both glutamate and glutamine using NMR is difficult as their spectra are entirely composed of coupled resonances and overlap with each other. Recently, CT-PRESS with optimized timing has been used to detect glutamate with a collapsed single peak. However, glutamine resonance was not resolved using this technique due to overlap with both glutamate and NAA. In this work, a method that achieves quantification of both glutamate and glutamine was proposed and applied to in-vivo CT-PRESS spectra.