SIMULTANEOUS QUANTIFICATION OF GLUTAMATE AND GLUTAMINE USING CT-PRESS AT 3T

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 谷氨酸和谷氨酰胺是人脑中的两种主要代谢物。谷氨酸和谷氨酰胺的准确定量对于了解大脑代谢至关重要，并且有可能揭示各种神经元疾病的机制。不幸的是，使用 NMR 定量谷氨酸和谷氨酰胺很困难，因为它们的光谱完全由耦合共振组成并且彼此重叠。最近，具有优化时序的 CT-PRESS 已被用于检测单峰塌陷的谷氨酸。然而，由于与谷氨酸和 NAA 重叠，因此使用该技术无法解决谷氨酰胺共振问题。在这项工作中，提出了一种实现谷氨酸和谷氨酰胺定量的方法，并将其应用于体内 CT-PRESS 谱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glutamate and glutamine are two major metabolites in human brain. Accurate quantification of both glutamate and glutamine is essential for understanding brain metabolism and has the potential to reveal mechanisms of various neuronal diseases. Unfortunately, quantification of both glutamate and glutamine using NMR is difficult as their spectra are entirely composed of coupled resonances and overlap with each other. Recently, CT-PRESS with optimized timing has been used to detect glutamate with a collapsed single peak. However, glutamine resonance was not resolved using this technique due to overlap with both glutamate and NAA. In this work, a method that achieves quantification of both glutamate and glutamine was proposed and applied to in-vivo CT-PRESS spectra.