SIMULTANEOUS QUANTIFICATION OF GLUTAMATE AND GLUTAMINE USING CT-PRESS AT 3T

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 谷氨酸和谷氨酰胺是人脑中的两种主要代谢物。谷氨酸和谷氨酰胺的准确定量对于了解大脑新陈代谢是必不可少的，并有可能揭示各种神经元疾病的机制。不幸的是，谷氨酸和谷氨酰胺的核磁共振定量是困难的，因为它们的光谱完全由耦合共振组成，并且相互重叠。最近，优化了时间的CT-PRESS被用于谷氨酸的单峰塌陷检测。然而，由于与谷氨酸和NAA重叠，使用该技术无法解析谷氨酰胺共振。在这项工作中，提出了一种同时实现谷氨酸和谷氨酰胺定量的方法，并将其应用于体内CT-PRESS谱。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glutamate and glutamine are two major metabolites in human brain. Accurate quantification of both glutamate and glutamine is essential for understanding brain metabolism and has the potential to reveal mechanisms of various neuronal diseases. Unfortunately, quantification of both glutamate and glutamine using NMR is difficult as their spectra are entirely composed of coupled resonances and overlap with each other. Recently, CT-PRESS with optimized timing has been used to detect glutamate with a collapsed single peak. However, glutamine resonance was not resolved using this technique due to overlap with both glutamate and NAA. In this work, a method that achieves quantification of both glutamate and glutamine was proposed and applied to in-vivo CT-PRESS spectra.