FIBER DIFFRACTION FROM FILAMENTOUS PLANT VIRUSES

丝状植物病毒的纤维衍射

• 批准号: 7954926
• 负责人: Gerald J Stubbs
• 金额: $ 0.87万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954926
• 关键词: Beet yellows virus; Biophysics; Biotechnology; Closteroviridae; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Family; Fiber; Filament; Funding; Grant; Hibiscus; Institution; Joints; Methods; Modeling; Plant Viruses; Potato; Potyviridae; Research; Research Personnel; Resolution; Resources; Sampling; Singapore; Source; Structure; Tobamovirus; United States National Institutes of Health; Viral; Virus; beamline; flexibility; method development; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are collecting fiber diffraction data from flexible filamentous plant viruses, invaluable as models for recognition, assembly, flexibility, viral-host interactions, and in methods development. Plant viruses have great potential in biotechnology, but development will require three-dimensional structures. Fiber diffraction is often the only method available for the determination of such filamentous structures. Diffraction samples are oriented sols and dried fibers. We find that order is better in dried fibers of highly flexible filaments, and in oriented sols of more rigid filaments. Dried fibers require much less material, an important consideration for studies of low-titer viruses. Wide and small angle diffraction data are collected at BioCAT; wide-angle data are also collected at BioCARS under a joint arrangement between the two beamlines. We collected wide-angle data on the filamentous plant viruses in August 2008. We obtained fiber diffraction data from potato virus Y (family Potyviridae), beet yellows virus (family Closteroviridae), and hibiscus latent Singapore virus (genus Tobamovirus). The HLSV data were particularly noteworthy, being the highest resolution (between 2.4 and 2.0, to be determined more accurately during later analysis) filamentous virus data that we have ever collected.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们正在收集来自柔性丝状植物病毒的纤维衍射数据，这些数据作为识别，组装，灵活性，病毒-宿主相互作用和方法开发的模型非常宝贵。植物病毒在生物技术方面具有巨大的潜力，但开发将需要三维结构。纤维衍射通常是唯一可用于确定这种丝状结构的方法。 衍射样品是定向溶胶和干燥纤维。我们发现，为了更好地在干纤维的高度灵活的长丝，并在更刚性的长丝取向溶胶。干燥的纤维需要更少的材料，这是研究低滴度病毒的重要考虑因素。宽角和小角衍射数据在BioCAT收集;宽角数据也在BioCARS在两条光束线之间的联合安排下收集。 我们在2008年8月收集了关于丝状植物病毒的广角数据。我们从马铃薯Y病毒（马铃薯Y病毒科）、甜菜黄化病毒（梭状病毒科）和木槿潜伏新加坡病毒（烟草花叶病毒属）获得了纤维衍射数据。HLSV数据特别值得注意，是我们曾经收集到的最高分辨率（在2.4和2.0之间，将在以后的分析中更准确地确定）丝状病毒数据。