FIBER DIFFRACTION FROM FILAMENTOUS PLANT VIRUSES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们正在收集柔性丝状植物病毒的纤维衍射数据，作为识别、组装、灵活性、病毒与宿主相互作用的模型以及方法开发的宝贵模型。植物病毒在生物技术方面有很大的潜力，但发展需要三维结构。纤维衍射通常是确定这种丝状结构的唯一方法。 衍射样品为取向的溶胶和干燥的纤维。我们发现，在高弹性长丝的干燥纤维中，以及在较坚硬的长丝的取向溶胶中，有序性较好。干燥的纤维需要的材料要少得多，这是研究低滴度病毒的重要考虑因素。在BioCAT收集广角和小角衍射数据；在BioCARS根据两条光束线的联合安排收集广角数据。 我们在2008年8月收集了关于丝状植物病毒的广角数据。我们获得了马铃薯Y病毒(马铃薯Y病毒科)、甜菜黄化病毒(新月形病毒科)和木槿花潜伏新加坡病毒(烟草病毒属)的纤维衍射数据。HLSV的数据尤其值得注意，它是我们收集过的丝状病毒数据中分辨率最高的(在2.4到2.0之间，将在以后的分析中更准确地确定)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are collecting fiber diffraction data from flexible filamentous plant viruses, invaluable as models for recognition, assembly, flexibility, viral-host interactions, and in methods development. Plant viruses have great potential in biotechnology, but development will require three-dimensional structures. Fiber diffraction is often the only method available for the determination of such filamentous structures. Diffraction samples are oriented sols and dried fibers. We find that order is better in dried fibers of highly flexible filaments, and in oriented sols of more rigid filaments. Dried fibers require much less material, an important consideration for studies of low-titer viruses. Wide and small angle diffraction data are collected at BioCAT; wide-angle data are also collected at BioCARS under a joint arrangement between the two beamlines. We collected wide-angle data on the filamentous plant viruses in August 2008. We obtained fiber diffraction data from potato virus Y (family Potyviridae), beet yellows virus (family Closteroviridae), and hibiscus latent Singapore virus (genus Tobamovirus). The HLSV data were particularly noteworthy, being the highest resolution (between 2.4 and 2.0, to be determined more accurately during later analysis) filamentous virus data that we have ever collected.