STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 SSRL Structural Molecular Biology小角X射线散射站BL 4 -2致力于结构生物学研究，为蛋白质、蛋白质组装体、病毒颗粒、生物纤维和脂质膜的结构生物学研究提供最先进的实验设施。2008年初夏，束线重建/升级到SPEAR 3 500 mA操作（包括新光学器件）完成，并在2008财年用户运行结束时进行了非常成功的调试运行。我们观察到高度改善的光束特性，特别是大大提高了光束的聚焦质量和更高的通量（一个因素的五），由于新的反射镜和全场摆动器操作，分别。我们还完成了箱内仪器的升级，配备了一台完全重建的针孔几何小角度散射相机，具有自动距离变化机制，可配置为适应多种数据收集模式，包括溶液散射，单晶衍射，纤维衍射和掠入射散射。该摄像机系统覆盖了2 A至2000 A范围内的特征长度尺度，具有七个离散的样品到检测器距离。一个Bonse-Hart几何超小角散射装置正在重新开发，以扩大范围高达几微米。新的CCD探测器（Rayonix MX225-HE）已收到并投入使用，并验证了其优异的特性，即更高的光子灵敏度和电子稳定性。我们继续开发生物x射线散射研究的软件，重点是高通量的解决方案x射线散射研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The SSRL Structural Molecular Biology small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for structural biology studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The beam line rebuild/upgrade to SPEAR3 500 mA operation, including new optics, was completed in early summer 2008 and had a highly successful commissioning run near the end of FY08 user run. We observed highly improved beam characteristics, in particular much improved beam focus quality and a higher flux (a factor of five) due to the new mirror and full-field wiggler operation, respectively. We also completed the upgrade of the in-hutch instrumentation, featuring a completely rebuilt pin-hole geometry small angle scattering camera with an automated distance change mechanism, which can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, fiber diffraction and gracing-incidence scattering. The camera system covers the characteristic length scale in the range of 2 A to 2000 A with seven discrete sample-to-detector distances. A Bonse-Hart geometry ultra-small angle scattering setup is being redeveloped to extend the range up to a few microns. A new CCD detector (Rayonix MX225-HE) was received and commissioned, and verified excellent characteristics, namely higher photon sensivity and electronic stability. We continue sofware developments for biological x-ray scattering studies with strong emphasis on high-throughput solution x-ray scattering studies.