STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

结构分子生物学小角度X射线散射站束线4-2

• 批准号: 8362069
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 8.22万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362069
• 关键词: Biological; Characteristics; Data Collection; Development; Environment; Fiber; Funding; Grant; In Situ; Incidence; Length; Lipids; Membrane Lipids; Molecular Biology; Monitor; National Center for Research Resources; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resources; Roentgen Rays; Sampling; Silicon; Solutions; Source; System; Temperature; Time; United States National Institutes of Health; Virion; X-Ray Crystallography; cost; detector; instrumentation; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The SSRL SMB small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for non-crystalline diffraction and scattering studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The in-hutch instrumentation features a variable-distance pin-hole geometry x-ray scattering camera system, which can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, lipid/fiber diffraction and gracing-incidence scattering. This system covers the characteristic length scale in the range of 2 A to 2000 A with seven discrete sample-to-detector distances. Advanced x-ray detectors, Rayonix MX225HE CCD and Dectris Pilatus 300K silicon pixel array detectors, are used for static and time-resolved projects, repsectively. The beam line has seen continued development of instrumentation for sample handling, in-situ monitoring of sample quality, and temperature control of sample environments.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 SSRL SMB小角度X射线散射站BL 4 -2致力于结构生物学研究，为蛋白质、蛋白质组装体、病毒颗粒、生物纤维和脂质膜的非晶体衍射和散射研究提供最先进的实验设施。该仪器具有可变距离针孔几何X射线散射相机系统，可以配置为适应多种数据收集模式，包括溶液散射，单晶衍射，脂质/纤维衍射和倾斜入射散射。该系统覆盖了2 A至2000 A范围内的特征长度标度，具有七个离散的样品至检测器距离。先进的X射线探测器，Rayonix MX225 HE CCD和Dectris Pilatus 300 K硅像素阵列探测器，分别用于静态和时间分辨项目。 光束线已经看到了持续发展的仪器样品处理，样品质量的现场监测，和样品环境的温度控制。