STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 SSRL SMB小角X射线散射站BL4-2致力于结构生物学研究，为蛋白质、蛋白质组件、病毒颗粒、生物纤维和脂膜的非晶态衍射和散射研究提供最先进的实验设备。内置式仪器配备了可变距离针孔几何X射线散射相机系统，该系统可配置为支持多种数据收集模式，包括溶液散射、单晶衍射、脂类/纤维衍射和光入射散射。该系统覆盖了2 A至2000 A范围内的特征长度范围，具有7个离散的样品到检测器距离。先进的X射线探测器Rayonix MX225HE和Dectris Pilatus 300K硅像素阵列探测器分别用于静态和时间分辨项目。光束线持续发展了用于样品处理、样品质量的现场监测和样品环境的温度控制的仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The SSRL SMB small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for non-crystalline diffraction and scattering studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The in-hutch instrumentation features a variable-distance pin-hole geometry x-ray scattering camera system, which can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, lipid/fiber diffraction and gracing-incidence scattering. This system covers the characteristic length scale in the range of 2 A to 2000 A with seven discrete sample-to-detector distances. Advanced x-ray detectors, Rayonix MX225HE CCD and Dectris Pilatus 300K silicon pixel array detectors, are used for static and time-resolved projects, repsectively. The beam line has seen continued development of instrumentation for sample handling, in-situ monitoring of sample quality, and temperature control of sample environments.