IRON K-BETA XES STUDIES OF THE NITROGENASE ENZYME SYSTEM AND MODELS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了了解Kbeta1，3(3p-1s)和Kbeta2，5(价态到1s)发射光谱随自旋态、氧化态和局域结构的变化，我们获得了一系列Fe(II)和Fe(III)模型配合物的K-βXES数据。特别令人感兴趣的是Kbeta？与配体2S到1S跃迁经验相关的交叉特征，因此可以用来区分碳、氮和氧。目前正在对这些数据进行分析。此外，还获得了固氮酶的MoFe蛋白和Delta-nifB突变体的初步数据，证明了这些方法用于稀蛋白体系研究的可行性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. K-beta XES data have been obtained on a series of Fe(II) and Fe(III) model complexes in order to develop an understanding of the changes which occur in the Kbeta1,3 (3p-1s) and Kbeta2,5 (valences to 1s) emission spectra as a function of spin state, oxidation state and local structure. Of particular interest are the Kbeta? crossover features which are empirically correlated to a ligand 2s to 1s transition, and as such may be used to differentiate carbon from nitrogen from oxygen. Analyses of these data are currently underway. In addition, preliminary data on the MoFe protein of nitrogenase and the delta-nifB mutant have been obtained and demonstrate the feasibility of these methods for investigation of dilute protein systems.