IRON K-BETA XES STUDIES OF THE NITROGENASE ENZYME SYSTEM AND MODELS

固氮酶系统和模型的铁 K-β XES 研究

• 批准号: 8169942
• 负责人: SERENA D DE BEER GEORGE
• 金额: $ 2.31万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169942
• 关键词: Biological Models; Carbon; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Enzymes; Funding; Grant; Institution; Investigation; Iron; Ligands; MYO5A gene; Methods; Modeling; Molybdoferredoxin; Nitrogen; Nitrogenase; Oxygen; Research; Research Personnel; Resources; Series; Source; Structure; System; United States National Institutes of Health; interest; mutant; oxidation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. K-beta XES data have been obtained on a series of Fe(II) and Fe(III) model complexes in order to develop an understanding of the changes which occur in the Kbeta1,3 (3p-1s) and Kbeta2,5 (valences to 1s) emission spectra as a function of spin state, oxidation state and local structure. Of particular interest are the Kbeta? crossover features which are empirically correlated to a ligand 2s to 1s transition, and as such may be used to differentiate carbon from nitrogen from oxygen. Analyses of these data are currently underway. In addition, preliminary data on the MoFe protein of nitrogenase and the delta-nifB mutant have been obtained and demonstrate the feasibility of these methods for investigation of dilute protein systems.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 已在一系列 Fe(II) 和 Fe(III) 模型配合物上获得了 K-beta XES 数据，以便了解 Kbeta1,3 (3p-1s) 和 Kbeta2,5（价态至 1s）发射光谱随自旋态、氧化态和局部结构而发生的变化。特别令人感兴趣的是 Kbeta？交叉特征在经验上与配体 2s 到 1s 的转变相关，因此可用于区分碳、氮和氧。目前正在对这些数据进行分析。此外，还获得了固氮酶 MoFe 蛋白和 delta-nifB 突变体的初步数据，证明了这些方法用于研究稀蛋白系统的可行性。