IRON K-BETA XES STUDIES OF THE NITROGENASE ENZYME SYSTEM AND MODELS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 已获得一系列Fe（II）和Fe（III）模型配合物的K β XES数据，以了解Kbeta 1，3（3 p-1 s）和Kbeta 2，5（价态至1 s）发射光谱中发生的变化作为自旋状态、氧化态和局部结构的函数。特别令人感兴趣的是Kbeta？经验上与配体2S到1 S跃迁相关的交叉特征，因此可用于区分碳、氮和氧。目前正在对这些数据进行分析。此外，初步数据的固氮酶和delta-nifB突变体的MoFe蛋白质已经获得，并证明了这些方法的可行性，用于调查稀释的蛋白质系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. K-beta XES data have been obtained on a series of Fe(II) and Fe(III) model complexes in order to develop an understanding of the changes which occur in the Kbeta1,3 (3p-1s) and Kbeta2,5 (valences to 1s) emission spectra as a function of spin state, oxidation state and local structure. Of particular interest are the Kbeta? crossover features which are empirically correlated to a ligand 2s to 1s transition, and as such may be used to differentiate carbon from nitrogen from oxygen. Analyses of these data are currently underway. In addition, preliminary data on the MoFe protein of nitrogenase and the delta-nifB mutant have been obtained and demonstrate the feasibility of these methods for investigation of dilute protein systems.