METAL ANALYSES OF BRAIN TISSUE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在BioCAT上，对阿尔茨海默病最常见的两个新皮质区域(有斑块和缠结)(额中背外侧)皮质(Brodman区46/9)和下颞叶皮质(Brodman区20)进行了金属含量分布图。 样本是在拉什大学医学中心的拉什阿尔茨海默病中心准备的。这些样本被认为是BSL2级。这项特殊实验的相应安全方案恰好提交给了生物安全委员会。专门为人脑组织设计的定制样品架可以容纳多达8个样品，上面覆盖着8微米厚的kapton薄膜。10微米厚的脑片安装在kapton薄膜上，并覆盖另一层kapton薄膜以保持它们的位置。样品保持器有两个保护性的亚克力幻灯片，当样品准备好在光束线上进行实验时，可以移除它们。当实验完成后，保护滑片被放回原位，以便将样品夹放回其容器中。 样品被暴露在微米尺寸的X射线束下，并对其金属含量进行评估。共测量了26例额叶中叶和6例小脑标本。采用Calmetrics的Fe、Cu、Zn标准溶液和NIST的Se、Hg标准溶液进行量化。每个样本的平均时间为1.5小时。通过连续扫描，扫描时间可以减少到10分钟/个样本。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metal content distribution mapping on two of the neocortical regions most commonly affected in Alzheimer's Disease, with both plaques and tangles, ( midfrontal dorsolateral) cortex (Brodman area 46/9) and the inferior temporal cortex (Brodman area 20) was performed at BioCAT. Samples were prepared at Rush Alzheimer Disease Center at Rush University Medical Center. These samples were considered BSL2 level. The corresponding safety protocol for this particular experiment was submitted to the Biosafety committee opportunely. A custom made sample holder, designed specifically for human brain tissue can hold up to 8 samples, covered with 8 micron thick kapton film. 10 microns thick brain slices were mounted on the kapton film and covered with another layer of kapton film to keep them in place. The sample holder has two protective acrylic slides that can be removed when the samples are ready for the experiment at the beamline. The protective slides were put back in place when the experiment is finished to handle the sample holder back to its container. Samples were exposed to the micron size x-ray beam and their metal content evaluated. A total of 26 midfrontal cortex samples and 6 cerebellum samples were measured. Fe, Cu and Zn standards from Calmetrics and Se and Hg standard solutions from NIST were used for quantization purposes. Average time per sample 1.5 hours. Scanning time can be reduce to 10 minutes/sample with continuous scans.