METAL ANALYSES OF BRAIN TISSUE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在BioCAT对阿尔茨海默病中最常受影响的两个新皮质区域（斑块和缠结）（中额叶背外侧）皮质（Brodman区域46/9）和下颞叶皮质（Brodman区域20）进行金属含量分布绘图。 在拉什大学医学中心的拉什阿尔茨海默病中心制备样品。 这些样品被认为是BSL 2水平。该特定实验的相应安全方案及时提交给了生物安全委员会。一个定制的样品保持器，专门为人脑组织设计，可以容纳多达8个样品，覆盖有8微米厚的Kapton膜。将10微米厚的脑切片固定在Kapton膜上，并用另一层Kapton膜覆盖以使它们保持在适当位置。样品保持器有两个保护性丙烯酸载玻片，当样品准备好在光束线上进行实验时，可以将其移除。 当实验完成时，将保护性载玻片放回原位，以将样品保持器处理回其容器。 将样品暴露于微米尺寸的X射线束，并评估其金属含量。共测量了26个中额叶皮质样本和6个小脑样本。 来自Calmetrics的Fe、Cu和Zn标准品以及来自NIST的Se和Hg标准品溶液用于量化目的。每个样本的平均时间为1.5小时。连续扫描的扫描时间可以减少到10分钟/样品。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metal content distribution mapping on two of the neocortical regions most commonly affected in Alzheimer's Disease, with both plaques and tangles, ( midfrontal dorsolateral) cortex (Brodman area 46/9) and the inferior temporal cortex (Brodman area 20) was performed at BioCAT. Samples were prepared at Rush Alzheimer Disease Center at Rush University Medical Center. These samples were considered BSL2 level. The corresponding safety protocol for this particular experiment was submitted to the Biosafety committee opportunely. A custom made sample holder, designed specifically for human brain tissue can hold up to 8 samples, covered with 8 micron thick kapton film. 10 microns thick brain slices were mounted on the kapton film and covered with another layer of kapton film to keep them in place. The sample holder has two protective acrylic slides that can be removed when the samples are ready for the experiment at the beamline. The protective slides were put back in place when the experiment is finished to handle the sample holder back to its container. Samples were exposed to the micron size x-ray beam and their metal content evaluated. A total of 26 midfrontal cortex samples and 6 cerebellum samples were measured. Fe, Cu and Zn standards from Calmetrics and Se and Hg standard solutions from NIST were used for quantization purposes. Average time per sample 1.5 hours. Scanning time can be reduce to 10 minutes/sample with continuous scans.