STRUCTURAL GENOMICS OF BACTERIAL PROTEINS

细菌蛋白质的结构基因组学

• 批准号: 7955112
• 负责人: WAYNE ANDERSON
• 金额: --
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955112
• 关键词: Amino Acid Sequence; Bacterial Proteins; Biochemical; Biological; Biology; Computer Retrieval of Information on Scientific Projects Database; Evolution; Family; Funding; Goals; Grant; Homologous Gene; Institution; Investigation; Large-Scale Sequencing; Lead; Ligands; Organism; Peptide Sequence Determination; Play; Protein Structure Initiative; Proteins; Research; Research Personnel; Resources; Role; Source; Structure; United States National Institutes of Health; genome sequencing; interest; member; protein structure; structural biology; structural genomics

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Comparison of protein sequences from completely sequenced genomes allows the clustering of the amino acid sequences into related families. The structures of the protein members of a sequence family will all be similar. While determining the structures of all proteins is an unreasonable aim, the determination of one structure from each sequence family is an attainable goal and the aim of the NIH Protein Structure Initiative. The large scale sequencing and structure determination efforts are going to provide extensive background information and lead to the question: What are the biological roles of these proteins? Those that have homologues in many widely divergent species must play an important role under some conditions in order to have been so widely retained through evolution and they are likely to have related functions in these diverse organisms. The sequencing efforts thus point out important proteins that are relatively uncharacterized and that require further investigation if we are to utilize the sequence information to expand our understanding of biology. The next logical step is to determine the biochemical functions of such proteins. We are interested in using protein structure to aid computational and experimental approaches to identifying possible ligands for proteins of unknown, or poorly defined function.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构为 研究中心，而研究中心不一定是研究者所在的机构。 比较来自完全测序的基因组的蛋白质序列允许将氨基酸序列聚类到相关家族中。序列家族的蛋白质成员的结构都是相似的。虽然确定所有蛋白质的结构是一个不合理的目标，但确定每个序列家族的一种结构是一个可以实现的目标，也是NIH蛋白质结构计划的目标。大规模的测序和结构测定工作将提供广泛的背景信息，并引出一个问题：这些蛋白质的生物学作用是什么？那些在许多差异很大的物种中具有同源物的基因在某些条件下一定发挥着重要作用，以便在进化过程中得到如此广泛的保留，并且它们很可能在这些不同的生物体中具有相关的功能。测序工作因此指出 这些重要的蛋白质相对来说还没有被鉴定，如果我们要利用序列信息来扩展我们对生物学的理解，就需要进一步的研究。下一个合乎逻辑的步骤是确定这些蛋白质的生化功能。我们感兴趣的是使用蛋白质的结构，以帮助计算和实验的方法来确定可能的配体蛋白质的未知，或定义不明确的功能。